The Analyst,
Journal Year:
2022,
Volume and Issue:
148(1), P. 9 - 25
Published: Dec. 7, 2022
Recent
years
have
witnessed
the
emergence
of
innovative
analytical
methods
with
high
sensitivity
and
spatiotemporal
resolution
that
allowed
qualitative
quantitative
analysis
to
be
carried
out
at
single-cell
subcellular
levels.
Electrochemiluminescence
(ECL)
is
a
unique
chemiluminescence
high-energy
electron
transfer
triggered
by
electrical
excitation.
The
ingenious
combination
electrochemistry
results
in
distinct
advantages
sensitivity,
wide
dynamic
range
good
reproducibility.
Specifically,
ECL
(SCECL)
excellent
has
emerged
as
promising
toolbox
bioanalysis
for
revealing
individual
cells'
heterogeneity
stochastic
processes.
This
review
focuses
on
advances
SCECL
bioimaging.
history
recent
probes
strategies
system
design
are
briefly
reviewed.
Subsequently,
latest
representative
techniques
bioassays,
bioimaging
therapeutics
also
highlighted.
Then,
current
challenges
future
perspectives
discussed.
Chemical Science,
Journal Year:
2020,
Volume and Issue:
11(38), P. 10496 - 10500
Published: Jan. 1, 2020
A
versatile
mechanism
based
on
a
chemical
lens
to
control
the
electrochemiluminescence
(ECL)
spatial
distribution
is
presented.
Changing
buffer
capacity
modifies
rate
of
ECL
reactions,
and
therefore
thickness
ECL-active
layer.
Molecular & Cellular Proteomics,
Journal Year:
2021,
Volume and Issue:
20, P. 100155 - 100155
Published: Jan. 1, 2021
Probing
the
human
proteome
in
tissues
and
biofluids
such
as
plasma
is
attractive
for
biomarker
drug
target
discovery.
Recent
breakthroughs
multiplex,
antibody-based,
proteomics
technologies
now
enable
simultaneous
quantification
of
thousands
proteins
at
low
sub
fg/ml
concentrations
with
remarkable
dynamic
ranges
up
to
10-log.
We
herein
provide
a
comprehensive
guide
methodologies,
performance,
technical
comparisons,
advantages,
disadvantages
established
emerging
multiplexed
ultrasensitive
measurement
proteins.
Gaining
holistic
knowledge
on
these
innovations
crucial
choosing
right
tool
applications
hand
critically
complement
traditional
methods.
This
can
bring
researchers
closer
than
ever
before
elucidating
intricate
inner
workings
cross
talk
that
spans
multitude
disease
mechanisms.
Biosensors,
Journal Year:
2020,
Volume and Issue:
10(9), P. 118 - 118
Published: Sept. 9, 2020
Electrogenerated
chemiluminescence
(also
called
electrochemiluminescence
(ECL))
has
become
a
great
focus
of
attention
in
different
fields
analysis,
mainly
as
consequence
the
potential
remarkably
high
sensitivity
and
wide
dynamic
range.
In
particular
case
sensing
applications,
ECL
biosensor
unites
benefits
selectivity
biological
recognition
elements
analysis
methods.
Hence,
it
is
powerful
analytical
device
for
sensitive
detection
analytes
interest
medical
prognosis
diagnosis,
food
control
environment.
These
range
applications
are
increased
by
introduction
screen-printed
electrodes
(SPEs).
Disposable
SPE-based
biosensors
cover
need
to
perform
in-situ
measurements
with
portable
devices
quickly
accurately.
this
review,
we
sum
up
latest
biosensing
current
progress
on
bioanalysis
combined
disposable
SPEs
field
bio
affinity
sensors
including
immunosensors,
DNA
catalytic
sensors.
Furthermore,
integration
nanomaterials
physical
chemical
properties
systems
improved
tremendously
their
overall
performance,
being
one
most
appropriates
research
development
highly
devices.
Analytical Chemistry,
Journal Year:
2021,
Volume and Issue:
93(23), P. 8152 - 8160
Published: June 3, 2021
Bipolar
electrochemistry
has
been
regarded
as
a
powerful
and
sustainable
electrochemical
process
for
the
synthesis
of
novel
functional
materials.
The
appealing
features
this
technology,
such
wireless
nature
bipolar
electrode
(BPE)
possibility
to
drive
simultaneously
reactions
on
multiple
BPEs
placed
in
same
cell,
together
with
change
shape
positioning
driving
electrodes,
give
significant
freedom
design
reaction
systems.
Nevertheless,
cell
geometry
dramatically
affects
distribution
intensity
potential
gradient
generated
BPE
surface
its
monitoring
is
hampered
due
BPE.
In
present
study,
we
propose
use
electrochemiluminescence
(ECL)
an
imaging
technique
map
cells
different
geometries.
proposed
approach
exploits
strong
ECL
emission
luminol/hydrogen
peroxide
(H2O2)
system
at
anodic
pole
BPE,
when
total
applied
voltage
(Etot)
enough
trigger
reaction.
Since
luminol
rather
intense
relatively
stable,
evolution
function
Etot
can
be
monitored
using
digital
camera,
allowing
elucidation
profile
every
configuration.
suggested
represents
valuable
reliable
method
systems
readily
employed
type
Analytical Chemistry,
Journal Year:
2021,
Volume and Issue:
93(49), P. 16425 - 16431
Published: Nov. 29, 2021
Electrochemiluminescence
(ECL)
behavior
of
luminol
derivative
was
investigated
in
reduction
on
different
electrode
materials.
We
found
that
and
its
widely
used
L-012
derivative,
emitting
at
physiological
pH
values,
exhibit
strong
cathodic
ECL
emission
iron
stainless
steel
electrodes
with
hydrogen
peroxide,
whereas
no
signal
observed
other
classic
materials
(Au,
Pt,
C).
On
a
Ni
electrode,
low
observed.
This
points
out
to
the
essential
role
iron-containing
enhance
emission.
Under
reported
conditions,
is
comparable
classically
anodic
Thus,
dual
bright
emissions
were
obtained
simultaneously
oxidation
as
imaged
wireless
bipolar
electrochemistry
configuration.
Such
an
system
generating
light
concomitantly
extremely
rare
it
opens
appealing
(bio)analytical
imaging
applications,
biosensing,
remote
detection,
analysis,
ECL-based
cell
microscopy.
ChemElectroChem,
Journal Year:
2022,
Volume and Issue:
9(12)
Published: April 25, 2022
Abstract
Sensors
rely
on
a
plurality
of
transduction
methods,
and
among
the
available
selections,
electrochemical
techniques
offer
peculiar
advantage
an
easy
interfacing
between
biological
recognition
elements
electronic
signal
processes.
Furthermore,
devices
are
qualified
for
decentralized
point‐of‐care
testing
as
they
can
be
easily
downsized
made
at
low
cost
wide
range
potential
applications,
example
biomedical
or
environmental.
Electrochemical
methods
differ
technique
used,
it
voltammetry,
chronoamperometry,
impedance
spectroscopy,
electrochemiluminescence.
The
latter
is
since
in
electrochemiluminescence
analytical
light
emission,
this
offers
several
advantages,
mainly
related
to
background
suppression
detection
limit,
combined
with
fast
measurement.
This
review
introduces
principles
enzyme
biosensors
coupled
transduction,
summarises
research,
finally
highlights
strategies
take
full
method.
Molecular & Cellular Proteomics,
Journal Year:
2023,
Volume and Issue:
22(6), P. 100557 - 100557
Published: April 23, 2023
Extracellular
vesicles
(EVs)
are
nanoscopic,
heterogenous,
lipid-rich
particles
that
carry
a
multitude
of
cargo
biomolecules
including
proteins,
nucleic
acids,
and
metabolites.
Although
historically
EVs
were
regarded
as
cellular
debris
with
no
intrinsic
value,
growing
understanding
EV
biogenesis
has
led
to
the
realization
facilitate
intercellular
communication
sources
liquid
biomarkers.
can
be
isolated
analyzed
from
wide
variety
accessible
biofluids
for
biomarker
discovery
diagnostic
applications.
There
is
diversity
different
biological
compartments
(e.g.,
cells
tissues),
some
these
present
at
extremely
low
concentrations.
Consequently,
challenge
in
field
find
appropriate
markers
enable
selective
isolation
rare
EVs.
Many
conventional
protein
detection
technologies
have
limited
sensitivity
detect
abundance
biomarkers
EVs,
limiting
their
use
research.
Advances
ultrasensitive
needed
harness
potential
clinical
application.
This
Perspective
highlights
current
research
focusing
on
technologies,
limitations,
areas
growth
future.
Microchimica Acta,
Journal Year:
2024,
Volume and Issue:
191(6)
Published: May 31, 2024
Electrogenerated
chemiluminescence
(ECL)
stands
out
as
a
remarkable
phenomenon
of
light
emission
at
electrodes
initiated
by
electrogenerated
species
in
solution.
Characterized
its
exceptional
sensitivity
and
minimal
background
optical
signals,
ECL
finds
applications
across
diverse
domains,
including
biosensing,
imaging,
various
analytical
applications.
This
review
aims
to
serve
comprehensive
guide
the
utilization
Beginning
with
brief
exposition
on
theory
basis
generation,
we
elucidate
systems
employed
initiate
ECL.
Furthermore,
delineate
principal
utilized
for
generation
contexts,
elucidating
both
advantages
challenges
inherent
their
use.
Additionally,
provide
an
overview
different
electrode
materials
novel
ECL-based
protocols
tailored
purposes,
specific
emphasis
biosensing
