Cytokine, Journal Year: 2025, Volume and Issue: 187, P. 156858 - 156858
Published: Jan. 18, 2025
Language: Английский
Advanced Functional Materials, Journal Year: 2024, Volume and Issue: 34(23)
Published: Jan. 7, 2024
Abstract The incidence of large bone and articular cartilage defects caused by traumatic injury is increasing worldwide; the tissue regeneration process for these injuries lengthy due to limited self‐healing ability. Endogenous bioelectrical phenomenon has been well recognized play an important role in homeostasis regeneration. Studies have reported that electrical stimulation (ES) can effectively regulate various biological processes holds promise as external intervention enhance synthesis extracellular matrix, thereby accelerating Hence, electroactive biomaterials considered a biomimetic approach ensure functional recovery integrating physiological signals, including electrical, biochemical, mechanical signals. This review will discuss endogenous bioelectricity tissue, effects ES on cellular behaviors. Then, recent advances materials their applications are systematically overviewed, with focus advantages disadvantages repair performances modulation cell fate. Finally, significance mimicking electrophysiological microenvironment target emphasized future development challenges strategies proposed.
Language: Английский
Citations
24
Journal of Nanobiotechnology, Journal Year: 2025, Volume and Issue: 23(1)
Published: Feb. 12, 2025
Senescent mandibular bone repair poses a formidable challenge without completely satisfactory strategy. Endogenous cell recruitment and osteogenic differentiation are two sequential stages in regeneration, disruptions these processes present significant obstacles to senescent repair. To address issues, engineered extracellular vesicles (EV) with stem functions were developed. This study demonstrated that Apt19s-engineered (Apt19s-EV) recognize recruit marrow mesenchymal cells derived from old rats (O-BMSCs) specifically effectively. MiR-376b-5p, identified by RNA sequencing transfection, was significantly decreased O-BMSCs, it selected construct miR-376b-5p-engineered (376b-EV). 376b-EV could promote osteogenesis alleviate senescence of O-BMSCs targeting Camsap1. combine the advantages Apt19s miR-376b-5p, dual (Apt-376b-EV) comprising both miR-376b-5p modifications constructed. further validate its function, Gelatin methacryloyl (GelMA) hydrogel used as carrier Apt-376b-EV@GelMA delivery system. The vitro results have sequentially. Notably, vivo also showed sequentially endogenous enhance new formation fracture critical-sized defect models. In summary, vesicles, Apt-376b-EV, offer an appealing solution for recruiting promoting microenvironment, which may broaden clinical applications EV provide valuable strategies treating bone-related diseases future work.
Language: Английский
Citations
2
Cell Division, Journal Year: 2025, Volume and Issue: 20(1)
Published: March 7, 2025
Language: Английский
Citations
2
International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(3), P. 1500 - 1500
Published: Jan. 28, 2022
Bone metabolism consists of a balance between bone formation and resorption, which is mediated by osteoblast osteoclast activity, respectively. In order to ensure plasticity, the remodeling process needs function properly. Mesenchymal stem cells differentiate into lineage activating different signaling pathways, including transforming growth factor β (TGF-β)/bone morphogenic protein (BMP) Wingless/Int-1 (Wnt)/β-catenin pathways. Recent data indicate that processes are also epigenetically regulated DNA methylation, histone post-translational modifications, non-coding RNA expressions, such as micro-RNAs, long RNAs, circular RNAs. Mutations dysfunctions in pathways regulating differentiation might influence process, ultimately leading large variety metabolic diseases. this review, we aim summarize describe genetics epigenetics process. Moreover, current findings behind diseases reported.
Language: Английский
Citations
50
Frontiers in Endocrinology, Journal Year: 2023, Volume and Issue: 14
Published: March 16, 2023
Diabetes has become a global public health problem. Diabetic foot is one of the most severe complications diabetes, which often places heavy economic burden on patients and seriously affects their quality life. The current conventional treatment for diabetic can only relieve symptoms or delay progression disease but cannot repair damaged blood vessels nerves. An increasing number studies have shown that mesenchymal stem cells (MSCs) promote angiogenesis re-epithelialization, participate in immune regulation, reduce inflammation, finally ulcer (DFU), rendering it an effective means treating disease. Currently, used are divided into two categories: autologous allogeneic. They mainly derived from bone marrow, umbilical cord, adipose tissue, placenta. MSCs different sources similar characteristics subtle differences. Mastering features to better select use premise improving therapeutic effect DFU. This article reviews types molecular mechanisms functions DFU provide innovative ideas using treat wound healing.
Language: Английский
Citations
37
Advanced Drug Delivery Reviews, Journal Year: 2024, Volume and Issue: 207, P. 115214 - 115214
Published: Feb. 22, 2024
Language: Английский
Citations
13
Current Issues in Molecular Biology, Journal Year: 2025, Volume and Issue: 47(3), P. 158 - 158
Published: Feb. 27, 2025
Purpose: The molecular mechanisms involved in bone metabolism abnormalities individuals with type 2 diabetes mellitus (T2DM) are a prominent area of investigation within the life sciences field. Myostatin (MSTN), member TGF-β superfamily, serves as critical negative regulator skeletal muscle growth and metabolism. Current research on exercise-mediated regulation MSTN expression predominantly focuses its role muscle. However, due to intricate multifaceted mechanical biochemical interactions between bone, precise by which exercise modulates enhance metabolic disorders T2DM necessitate additional exploration. objective this review is systematically synthesize evaluate development associated elucidate underlying influenced interventions, aiming offer novel insights theoretical recommendations for enhancing health through physical activity. Methods: Relevant articles Chinese English up July 2024 were selected using specific search terms databases (PubMed, CNKI, Web Science); 147 studies finally included after evaluation, reference lists checked other relevant research. Results: Myostatin’s heightened can impede various pathways, such PI3K/AKT/mTOR Wnt/β-catenin, hindering osteoblast differentiation mineralization. Additionally, it stimulate osteoclast resorption capacity facilitating Smad2-dependent NFATc1 nuclear translocation PI3K/AKT/AP-1-mediated pro-inflammatory factor thereby contributing disorders. Physical plays crucial ameliorating T2DM. Exercise activate pathways like Wnt/GSK-3β/β-catenin, suppressing myostatin downstream Smads, CCL20/CCR6, Nox4 target gene expression, fostering formation, inhibiting resorption, Conclusion: In context T2DM, has been shown exacerbate osteoblasts process mineralization while simultaneously promoting activity osteoclasts. interventions have demonstrated efficacy downregulating disrupting signaling
Language: Английский
Citations
1
Journal of Orthopaedic Translation, Journal Year: 2025, Volume and Issue: 51, P. 177 - 186
Published: March 1, 2025
Language: Английский
Citations
1
International Journal of Molecular Sciences, Journal Year: 2021, Volume and Issue: 22(11), P. 6104 - 6104
Published: June 5, 2021
Melanogenesis is the process leading to synthesis of melanin, main substance that influences skin color and plays a pivotal role against UV damage. Altered melanogenesis observed in several pigmentation disorders. occurs specialized cells called melanocytes, physically functionally related by means autocrine paracrine interplay other cell types. Several external internal factors control melanin biosynthesis operate through different intracellular signaling pathways, which finally leads regulation microphthalmia-associated transcription factor (MITF), key involved expression melanogenic enzymes, including TYR, TYRP-1, TYRP-2. Epigenetic factors, microRNAs (miRNAs), are regulation. miRNAs small, single-stranded, non-coding RNAs, approximately 22 nucleotides length, behavior regulating gene expression, mainly binding 3' untranslated region (3'-UTR) target mRNAs. This review collects data on how these can modulate expression. Bringing light biological function could lead wider understanding epigenetic its dysregulation. knowledge may constitute basis for developing innovative treatment approaches
Language: Английский
Citations
50
Small, Journal Year: 2023, Volume and Issue: 20(16)
Published: Nov. 28, 2023
Abstract The study investigated whether both the osteogenic and angiogenic potential of Exos (Exosomes) can be enhanced by overexpression exosomal miRNA (microRNA) to confirm loaded in HMPs (Hydrogel microparticles) exert long‐term effects during new bone formation. BMSCs are successfully obtained. In vitro vivo experiments confirmed that HDAC4 (Histone deacetylase 4) is inhibited miR‐29a accompanied upregulation RUNX2 (Runt‐related transcription factor 2) VEGF (Vascular Endothelial Growth Factor), thereby enhancing capabilities. HMP@Exo system synthesized from HB‐PEGDA (Hyperbranched Poly Ethylene Glycol Diacrylate)‐ SH‐HA (Sulfhydryl‐Modified Hyaluronic Acid)‐containing using a microfluidic technique. HMP surface modified with RGD (Arg‐Gly‐Asp) peptides enhance cell adhesion. demonstrated good injectability, remarkable compatibility, outstanding adhesion properties, slow degradation capacity, sustained release Agomir‐29a‐Exos (Exosomes derived Agomir‐29a transfected BMSCs) proliferation migration HUVECs (Human Umbilical Vein Cells) while promoting osteogenesis angiogenesis. Overall, findings demonstrate effectively maintain activity half‐life Exos, (microRNA‐29a). injectable provides an innovative approach for accelerating fracture healing coupling
Language: Английский
Citations
22