ACS Applied Nano Materials, Journal Year: 2025, Volume and Issue: unknown
Published: March 25, 2025
Language: Английский
Advanced Materials, Journal Year: 2024, Volume and Issue: 36(21)
Published: Feb. 3, 2024
Abstract Spinal cord injury (SCI) is a refractory neurological disorder. Due to the complex pathological processes, especially secondary inflammatory cascade and lack of intrinsic regenerative capacity, it difficult recover function after SCI. Meanwhile, simulating conductive microenvironment spinal reconstructs electrical neural signal transmission interrupted by SCI facilitates repair. Therefore, double‐crosslinked hydrogel (BP@Hydrogel) containing black phosphorus nanoplates (BP) synthesized. When placed in rotating magnetic field (RMF), BP@Hydrogel can generate stable signals exhibit electrogenic characteristic. In vitro, shows satisfactory biocompatibility alleviate activation microglia. RMF, enhances anti‐inflammatory effects. wireless stimulation promotes differentiation stem cells (NSCs) into neurons, which associated with PI3K/AKT pathway. vivo, injectable elicit behavioral electrophysiological recovery complete transected mice alleviating inflammation facilitating endogenous NSCs form functional neurons synapses under RMF. The present research develops multifunctional for repair targeting multiple mechanisms including immunoregulation enhancement neuronal differentiation.
Language: Английский
Citations
37
Stem Cell Research & Therapy, Journal Year: 2024, Volume and Issue: 15(1)
Published: Jan. 2, 2024
Abstract Spinal cord injury (SCI) is a catastrophic to the central nervous system (CNS) that can lead sensory and motor dysfunction, which seriously affects patients' quality of life imposes major economic burden on society. The pathological process SCI divided into primary secondary injury, cascade amplified responses triggered by injury. Due complexity mechanisms SCI, there no clear effective treatment strategy in clinical practice. Exosomes, are extracellular vesicles endoplasmic origin with diameter 30–150 nm, play critical role intercellular communication have become an ideal vehicle for drug delivery. A growing body evidence suggests exosomes great potential repairing SCI. In this review, we introduce exosome preparation, functions, administration routes. addition, summarize effect mechanism various repair review efficacy combination other strategies Finally, challenges prospects use described.
Language: Английский
Citations
18
ACS Nano, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 27, 2025
Extracellular vesicles (EVs) are cell derived nanovesicles which implicated in both physiological and pathological intercellular communication, including the initiation, progression, metastasis of cancer. The exchange biomolecules between stromal cells cancer via EVs can provide a window to monitor development real time for better diagnostic interventional strategies. In addition, process secretion internalization by tumor microenvironment (TME) be exploited delivering therapeutics. have potential targeted, biocompatible, efficient delivery platform treatment other diseases. Natural as well engineered nanomedicine immense disease intervention. Here, we an overview current knowledge EVs' function therapeutic applications setting, EV engineering
Language: Английский
Citations
3
Advanced Science, Journal Year: 2023, Volume and Issue: 10(31)
Published: Sept. 22, 2023
Modulating the inflammatory microenvironment can inhibit process of diseases (IDs). A tri-cross-linked microenvironment-responsive hydrogel with ideal mechanical properties achieves triggerable and sustained drug delivery regulates microenvironment. Here, this study develops an (OD-PP@SeNPs) composed phenylboronic acid grafted polylysine (PP), oxidized dextran (OD), selenium nanoparticles (SeNPs). The introduction SeNPs as initiators nano-fillers into results in extra cross-linking polymer network through hydrogen bonding. Based on Schiff base bonds, Phenylboronate ester a reactive oxygen species (ROS)/pH dual responsive triple-network is achieved. has injectable, self-healing, adhesion, outstanding flexibility, suitable swelling capacity, optimal biodegradability, excellent stimuli-responsive active substance release performance, prominent biocompatibility. Most importantly, ROS scavenging pH-regulating ability protects cells from oxidative stress induces macrophages M2 polarization to reduce cytokines PI3K/AKT/NF-κB MAPK pathways, exerting anti-inflammatory effects reshaping microenvironment, thereby effectively treating typical IDs, including S. aureus infected wound rheumatoid arthritis rats. In conclusion, dynamically injectable structure provides effective strategy treat holding great promise clinical application.
Language: Английский
Citations
41
ACS Omega, Journal Year: 2023, Volume and Issue: 8(46), P. 43374 - 43387
Published: Nov. 9, 2023
Exosomes are nanoscale vesicles secreted by living cells that have similar membrane composition to parental and carry a variety of proteins, lipids, nucleic acids. Therefore, exosomes certain biological activities play an important role in intercellular communication. On the basis its potential as carrier for drug delivery systems, been engineered compensate shortage natural through various engineering strategies improving efficiency, enhancing targeting tissues organs, extending circulating half-life exosomes. This review focuses on loading drugs different strategies, discussions exosome surface modification summarizes advantages disadvantages strategies. In addition, this provides overview recent applications number refractory relapsable diseases. has provide reference further research development
Language: Английский
Citations
36
Bioactive Materials, Journal Year: 2024, Volume and Issue: 38, P. 438 - 454
Published: May 12, 2024
Spinal cord injury (SCI) is a traumatic condition that results in impaired motor and sensory function. Ferroptosis one of the main causes neural cell death loss neurological function spinal cord, ferroptosis inhibitors are effective reducing inflammation repairing SCI. Although human umbilical mesenchymal stem cells (Huc-MSCs) can ameliorate inflammatory microenvironments promote regeneration SCI, their efficacy greatly limited by local microenvironment after Therefore, this study, we constructed drug-release nanoparticle system with synergistic Huc-MSCs inhibitor, which anchored Tz-A6 peptide based on CD44-targeting sequence, combined reactive oxygen species (ROS)-responsive drug nanocarrier mPEG-b-Lys-BECI-TCO at other end for SCI repair. Meanwhile, also modified classic inhibitor Ferrostatin-1 (Fer-1) synthesized new prodrug Feborastatin-1 (Feb-1). The showed treatment regimen significantly inhibited response promoted recovery rats This study developed combination therapy provides strategy construction drug-coordinated system.
Language: Английский
Citations
15
International Journal of Nanomedicine, Journal Year: 2024, Volume and Issue: Volume 19, P. 1597 - 1627
Published: Feb. 1, 2024
Abstract: The development of numerous diseases is significantly influenced by inflammation. Macrophage-derived exosomes (M-Exos) play a role in controlling inflammatory reactions various conditions, including chronic pain, hypertension, and diabetes. However, the specific targets roles M-Exos regulating inflammation remain largely unknown. This review summarizes current knowledge on biogenesis provides updated information M-Exos' biological function modulation. Furthermore, this highlights functionalization engineering strategies M-Exos, while providing an overview cutting-edge approaches to advancements their application as therapeutics for Finally, multiple mechanisms are presented along with perspectives challenges, potential contribution that may have through modulation discussed. Keywords: inflammation, macrophage-derived exosomes, functions, modulation,
Language: Английский
Citations
14
European Journal of Pharmacology, Journal Year: 2025, Volume and Issue: unknown, P. 177236 - 177236
Published: Jan. 1, 2025
Language: Английский
Citations
1
International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(2), P. 723 - 723
Published: Jan. 16, 2025
Mesenchymal stem cell-derived extracellular vesicles (MSC-EVs) have emerged as a promising therapeutic strategy for spinal cord injury (SCI). These nanosized possess unique properties such low immunogenicity and the ability to cross biological barriers, making them ideal carriers delivering bioactive molecules injured tissues. MSC-EVs been demonstrated exert multiple beneficial effects in SCI, including reducing inflammation, promoting neuroprotection, enhancing axonal regeneration. Recent studies delved into molecular mechanisms underlying MSC-EV-mediated effects. Exosomal microRNAs (miRNAs) identified key regulators of various cellular processes involved SCI pathogenesis repair. miRNAs can influence oxidative stress, apoptosis by modulating gene expression. This review summarized current state MSC-EV-based therapies highlighting potential clinical applications. We discussed challenges limitations translating these practice, inconsistent EV production, complex cargo composition, need targeted delivery strategies. Future research should focus on optimizing production characterization, identifying miRNAs, developing innovative systems maximize SCI.
Language: Английский
Citations
1
ACS Nano, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 25, 2025
Extracellular vesicles (EVs) hold promise for tissue regeneration, but their low yield and limited therapeutic efficacy hinder clinical translation. Bioreactors provide a larger culture surface area stable environment large-scale EV production, yet ability to enhance is limited. Physical stimulation, by inducing cell differentiation modulating cargo composition, offers more efficient, cost-effective, reproducible approach compared the loading of EVs biochemical priming parental cells. Herein, effects 3D-printed perfusion bioreactor with topographical cue on macrophage bioactivity were assessed. The results indicate that increased 12.5-fold enhanced in promoting osteogenic angiogenesis via upregulated miR-210-3p. Mechanistically, fluid shear stress activates Piezo1, triggering Ca2+ influx Yes-associated protein (YAP) nuclear translocation, secretion enhancing M2 polarization conjunction morphological changes guided aligned topography. Moreover, porous electrospun membrane-hydrogel composite scaffold loaded bioreactor-derived exhibited outstanding rat cranial defect model. This study presents scalable, platform production EVs, potentially overcoming key challenges translating EV-based therapies clinic.
Language: Английский
Citations
1