Mercury(II)-Triggered Targeted and NIR-II Fluorescence/Photoacoustic Imaging Probe for High-Sensitivity Early Diagnosis and Evaluating Drug against Acute Liver and Kidney Injury DOI
Xinyue Li, Zhongxia Duan, Zhi‐Wen Zhao

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 6, 2025

Mercury ions (Hg2+) have been found to disrupt the body's antioxidant defense mechanisms, leading oxidative stress and physiological dysfunction. Early diagnosis real-time monitoring of Hg2+ fluctuations in organ damage are crucial but limited due lack noninvasive deep tissue imaging probes. Herein, a Hg2+-triggered targeted NIR-II fluorescence/photoacoustic (PA) dual-mode molecular probe (NHG-2) was developed for Hg2+-induced acute liver kidney injury mice. NHG-2 designed through rational adjustment conjugated ring structure further screening processes, enabling it sensitively recognize subsequently open mitochondrial targeting, producing fluorescence/PA signals. This allowed injury, demonstrating excellent detection sensitivity. Furthermore, can be utilized evaluate efficacy N-acetylcysteine (NAC) dual signal indication. Mechanism studies suggested that NAC activated Akt/Nrf2 signaling pathway, reversed changes related biomarkers, restored membrane potential. Thus, this study not only presents first specific also provides potential tool early treatment evaluation pathogenesis study.

Language: Английский

Near-infrared luminescence high-contrast in vivo biomedical imaging DOI Open Access
Ying Chen, Shangfeng Wang, Fan Zhang

et al.

Nature Reviews Bioengineering, Journal Year: 2023, Volume and Issue: 1(1), P. 60 - 78

Published: Jan. 19, 2023

Language: Английский

Citations

277
Recent progress in the development of fluorescent probes for imaging pathological oxidative stress DOI Creative Commons

Yujie Geng,

Zhuo Wang,

Jiaying Zhou

et al.

Chemical Society Reviews, Journal Year: 2023, Volume and Issue: 52(11), P. 3873 - 3926

Published: Jan. 1, 2023

Oxidative stress is closely related to the physiopathology of numerous diseases. Reactive oxygen species (ROS), reactive nitrogen (RNS), and sulfur (RSS) are direct participants important biomarkers oxidative stress.

Language: Английский

Citations

161
Stable, Bright, and Long-Fluorescence-Lifetime Dyes for Deep-Near-Infrared Bioimaging DOI
Jin Li, Yan Dong,

Ruwei Wei

et al.

Journal of the American Chemical Society, Journal Year: 2022, Volume and Issue: 144(31), P. 14351 - 14362

Published: July 29, 2022

Near-infrared (NIR) fluorophores absorbing maximally in the region beyond 800 nm, i.e., deep-NIR spectral region, are actively sought for biomedical applications. Ideal dyes bright, nontoxic, photostable, biocompatible, and easily derivatized to introduce functionalities (e.g., bioconjugation or aqueous solubility). The rational design of such remains a major challenge. Silicon-substituted rhodamines have been successful bioimaging applications red region. longer-wavelength silicon-substituted congeners unknown date. We successfully prepared four bis-benzannulated rhodamine (ESi5a–ESi5d), with an efficient five-step cascade on gram-scale. Because extensive overlapping their HOMO–LUMO orbitals, ESi5a–ESi5d highly (λabs ≈ 865 nm ε > 105 cm–1 M–1). By restraining both rotational freedom via annulation vibrational silicon-imparted strain, fluorochromic scaffold ESi5 is rigid, resulting unusually long fluorescence lifetime (τ 700 ps CH2Cl2) high quantum yield (ϕ = 0.14 CH2Cl2). Their half-lives toward photobleaching 2 orders magnitude longer than current standard (ICG serum). They stable presence biorelevant concentration nucleophiles reactive oxygen species. minimally toxic readily metabolized. Upon tail vein injection ESi5a (as example), vasculature nude mouse was imaged signal-to-background ratio. broad potentials

Language: Английский

Citations

98
NIRII‐HDs: A Versatile Platform for Developing Activatable NIR‐II Fluorogenic Probes for Reliable In Vivo Analyte Sensing DOI

Zuojia Qin,

Tian‐Bing Ren, Huijie Zhou

et al.

Angewandte Chemie International Edition, Journal Year: 2022, Volume and Issue: 61(19)

Published: Feb. 26, 2022

Small-molecule-based second near-infrared (NIR-II) activatable fluorescent probes can potentially provide a high target-to-background ratio and deep tissue penetration. However, most of the reported NIR-II small-molecule exhibit poor versatility owing to lack general stable optically tunable group. In this study, we designed NIRII-HDs, novel dye scaffold optimized for probe development. particular, NIRII-HD5 showed best optical properties such as proper pKa value, excellent stability, brightness, which be beneficial in vivo imaging with contrast. To demonstrate applicability dye, three target-activatable ROS, thiols, enzymes. Using these probes, not only realized reliable different diseases mouse models but also evaluated redox potential liver during injury fidelity.

Language: Английский

Citations

94
Design of NIR-II high performance organic small molecule fluorescent probes and summary of their biomedical applications DOI
Xinyu Liu, Bing Yu, Youqing Shen

et al.

Coordination Chemistry Reviews, Journal Year: 2022, Volume and Issue: 468, P. 214609 - 214609

Published: May 31, 2022

Language: Английский

Citations

93
Counterion‐Paired Bright Heptamethine Fluorophores with NIR‐II Excitation and Emission Enable Multiplexed Biomedical Imaging DOI
Yang Yang,

Caixia Sun,

Shangfeng Wang

et al.

Angewandte Chemie International Edition, Journal Year: 2022, Volume and Issue: 61(24)

Published: March 16, 2022

Abstract Photon excitation and emission at the NIR‐II spectral window enable high‐contrast deep‐tissue bioimaging. However, multiplexed imaging with has been hampered by limited chemical strategies to develop bright fluorophores tunable absorption in this regime. Herein, we developed a series of heptamethine cyanines (HCs) varied absorption/emission maxima spanning from 1100 1600 nm through physical organic approach. A bulky counterion paired HCs was found elicit substantial improvements absorptivity (7‐fold), brightness (14‐fold), profiles water, addressing notorious quenching problem due aggregation polarization. We demonstrated utilities HC1222 HC1342 for dual‐color circulatory system, lymphatic structures, tumor, organ function living mice under 1120 1319 excitation, showing as promising platform non‐invasive

Language: Английский

Citations

84
Engineering of Reversible NIR‐II Redox‐Responsive Fluorescent Probes for Imaging of Inflammation In Vivo DOI
Long He,

Linhui He,

Shuai Xu

et al.

Angewandte Chemie International Edition, Journal Year: 2022, Volume and Issue: 61(46)

Published: Sept. 23, 2022

The second near-infrared (NIR-II) fluorescent imaging shows great potential for deep tissue analysis at high resolution in living body owing to low background autofluorescence and photon scattering. However, reversible monitoring of redox homeostasis using NIR-II remains a challenge due the lack appropriate probes. In this study, series stable multifunctional dyes (NIR-II Cy3s) were constructed based on trimethine skeleton. Significantly, introducing 1,4-diethyl-decahydroquinoxaline group Cy3s not only effectively increased wavelength, but also served as an effective response site HClO, which can be restored by reactive sulfur species (RSS). Based this, used HClO/RSS-mediated processes pathophysiology environment. Finally, Cy3-988 was successfully utilized assessment environments drug treatment effects acute inflammation model.

Language: Английский

Citations

82
The pursuit of polymethine fluorophores with NIR-II emission and high brightness forin vivoapplications DOI Creative Commons
Xuan Zhao, Fan Zhang, Zuhai Lei

et al.

Chemical Science, Journal Year: 2022, Volume and Issue: 13(38), P. 11280 - 11293

Published: Jan. 1, 2022

Polymethine cyanine dyes, as the most important class of organic near-infrared-II (NIR-II) fluorophores, recently received increasing attention due to their high molar extinction coefficients, intensive fluorescence brightness, and flexible wavelength tunability for fluorescent bioimaging applications. Very recently, remarkable advances have been made in development NIR-II polymethine fluorophores with improved optical performance, mainly including tunable fluorescence, water solubility stability. In this review, we summarize recent research molecular tailoring design strategies then emphasize representative biosensing The potential challenges perspectives emerging field are also discussed. This review may provide guidance reference further high-performance boost clinical translation future.

Language: Английский

Citations

78
Polymethine Molecular Platform for Ratiometric Fluorescent Probes in the Second near-Infrared Window DOI
Qingchun Lan, Peng Yu,

Kui Yan

et al.

Journal of the American Chemical Society, Journal Year: 2022, Volume and Issue: 144(46), P. 21010 - 21015

Published: Oct. 25, 2022

Visualizing biomolecules such as enzymes in the deep tissue of living organisms via molecular ratiometric fluorescent probes second near-infrared window (NIR-II) with a built-in self-calibration function can provide reliable information about relevant pathophysiological processes directly but so far is not feasible due to lack fluorescence modulation strategy NIR-II window. Here we present platform Py-2 by integrating rhodamine 6G scaffold and polymethine. The maximal emission wavelength was 1010 nm blue-shifted 945 when its secondary amine acylated. Based on Py-2, two probes, nitroreductase-responsive Rap-N ROS-responsive Rap-R, were constructed successfully demonstrated vitro vivo. Overall, this report presents unique approach developing for vivo biosensing.

Language: Английский

Citations

75
Activatable NIR-II organic fluorescent probes for bioimaging DOI Creative Commons
Xiaoning Zhang, Shasha Li, Huizhen Ma

et al.

Theranostics, Journal Year: 2022, Volume and Issue: 12(7), P. 3345 - 3371

Published: Jan. 1, 2022

NIR-II imaging is developed rapidly for noninvasive deep tissue inspection with high spatio-temporal resolution, taking advantage of diminished autofluorescence and light attenuation.Activatable fluorescence probes are widely to report pathological changes accurate targeting, among which organic fluorescent achieve significant progress.Furthermore, the activatable exhibited appealing characteristics like tunable physicochemical optical properties, easy processability, excellent biocompatibility.In present review, we highlight advances in design, synthesis applications reactive oxygen species (ROS), nitrogen (RNS), sulfur (RSS), pH, hypoxia, viscosity as well abnormally expressed enzymes.This non-invasive modality shows a promising prospect targeting site envisioned potential clinical translation.

Language: Английский

Citations

74