Impact of Point-of-Care Testing on Diagnosis, Treatment, and Surveillance of Vaccine-Preventable Viral Infections DOI Creative Commons

Kirthika Lakshmanan,

Baoming Liu

Diagnostics, Journal Year: 2025, Volume and Issue: 15(2), P. 123 - 123

Published: Jan. 7, 2025

With the advent of a variety vaccines against viral infections, there are multiple viruses that can be prevented via vaccination. However, breakthrough infections or uncovered strains still cause vaccine-preventable (VPVIs). Therefore, timely diagnosis, treatment, and surveillance these is critical to patient care public health. Point-of-care (POC) diagnostics tools have brought significant improvements in detection management VPVIs. These cutting-edge technologies enable prompt accurate results, enhancing by facilitating treatment decisions. This review delves into advancements POC testing, including antigen/antibody molecular assays, while focusing on their impact VPVIs such as mpox, hepatitis, influenza, flaviviruses (dengue, Zika, yellow fever virus), COVID-19. The role tests monitoring infection crucial for tracking disease progression managing outbreaks. Furthermore, application has shown vital health strategies. In this review, we also highlight emerging CRISPR-based smartphone-integrated devices, which proven particularly beneficial resource-limited settings. We underscore importance continued research optimize diagnostic wider global use dengue, COVID-19, addressing current challenges related sensitivity, specificity, availability, efficiency, more.

Language: Английский

New challenges in point of care electrochemical detection of clinical biomarkers DOI
Susana Campuzano, Marı́a Pedrero, Paloma Yáñez‐Sedeño

et al.

Sensors and Actuators B Chemical, Journal Year: 2021, Volume and Issue: 345, P. 130349 - 130349

Published: June 25, 2021

Language: Английский

Citations

110
Signal amplification and output of CRISPR/Cas-based biosensing systems: A review DOI
Siyuan Wang, Yichen Du, Dongxia Wang

et al.

Analytica Chimica Acta, Journal Year: 2021, Volume and Issue: 1185, P. 338882 - 338882

Published: July 26, 2021

Language: Английский

Citations

109
CRISPR‐Cas Biochemistry and CRISPR‐Based Molecular Diagnostics DOI Open Access

Zhengyan Weng,

Zheng You, Jie Yang

et al.

Angewandte Chemie International Edition, Journal Year: 2023, Volume and Issue: 62(17)

Published: Jan. 30, 2023

Abstract Polymerase chain reaction (PCR)‐based nucleic acid testing has played a critical role in disease diagnostics, pathogen surveillance, and many more. However, this method requires long turnaround time, expensive equipment, trained personnel, limiting its widespread availability diagnostic capacity. On the other hand, clustered regularly interspaced short palindromic repeats (CRISPR) technology recently demonstrated capability for detection with high sensitivity specificity. CRISPR‐mediated biosensing holds great promise revolutionizing procedures developing point‐of‐care diagnostics. This review focuses on recent developments both fundamental CRISPR biochemistry CRISPR‐based techniques. Four ongoing research hotspots molecular diagnostics‐target preamplification‐free detection, microRNA (miRNA) testing, non‐nucleic‐acid SARS‐CoV‐2 detection‐are also covered.

Language: Английский

Citations

105
A novel fluorescence biosensor based on CRISPR/Cas12a integrated MXenes for detecting Aflatoxin B1 DOI Creative Commons
Zhihui Wu, Da‐Wen Sun, Hongbin Pu

et al.

Talanta, Journal Year: 2022, Volume and Issue: 252, P. 123773 - 123773

Published: Aug. 6, 2022

Aflatoxin B1 (AFB1) contamination in food threatens global safety, and rapid quantitative detection of AFB1 remains a challenge. Herein, novel fluorescence biosensor was developed for based on CRISPR/Cas12a MXenes. Specifically, the well-designed activator locked by dual-AFB1 aptamers, Cas12a directly linked to crRNA form inactivated complexes, MXenes efficiently adsorbed FAM fluorophore-modified single-stranded DNA (ssDNA-FAM), quenching its fluorescence. In presence AFB1, released due preferential binding aptamer then activated trans-cleavage activity indiscriminately cleave ssDNA MXenes, leading recovery signal. The fluorescent had wide range from 0.001 80 ng mL-1, limit 0.92 pg ability detect within min. More importantly, platform demonstrates excellent performance real peanut samples.

Language: Английский

Citations

94
Towards application of CRISPR-Cas12a in the design of modern viral DNA detection tools (Review) DOI Creative Commons

Julija Dronina,

Urtė Samukaitė-Bubnienė, Arūnas Ramanavičius

et al.

Journal of Nanobiotechnology, Journal Year: 2022, Volume and Issue: 20(1)

Published: Jan. 21, 2022

Abstract Early detection of viral pathogens by DNA-sensors in clinical samples, contaminated foods, soil or water can dramatically improve outcomes and reduce the socioeconomic impact diseases such as COVID-19. Clustered regularly interspaced short palindromic repeat (CRISPR) its associated protein Cas12a (previously known CRISPR-Cpf1) technology is an innovative new-generation genomic engineering tool, also ‘genetic scissors’, that has demonstrated accuracy recently been effectively applied appropriate (E-CRISPR) DNA-sensor to detect nucleic acid interest. The CRISPR-Cas12a from Prevotella Francisella 1 are guided a CRISPR RNA (gRNA). unique simultaneous cis- trans- DNA cleavage after target sequence recognition at PAM site, sticky-end (5–7 bp) employment, ssDNA/dsDNA hybrid strategies manipulate attractive nature CRISPR–Cas12a reviewed. based on for rapid, robust, sensitive, inexpensive, selective virus without additional sample purification, amplification, fluorescent-agent- and/or quencher-labeling relevant becoming increasingly important industrial medical applications. In addition, system shows great potential field E-CRISPR-based bioassay research technologies. Therefore, we highlighting insights this direction. Graphical

Language: Английский

Citations

92
Emerging Biosensing Technologies for the Diagnostics of Viral Infectious Diseases DOI Creative Commons
Gözde Kabay, Jonalyn DeCastro,

Alara Altay

et al.

Advanced Materials, Journal Year: 2022, Volume and Issue: 34(30)

Published: March 14, 2022

Abstract Several viral infectious diseases appear limitless since the beginning of 21 st century, expanding into pandemic lengths. Thus, there are extensive efforts to provide more efficient means diagnosis, a better understanding acquired immunity, and improved monitoring inflammatory biomarkers, as these all crucial for controlling spread infection while aiding in vaccine development improving patient outcomes. In this regard, various biosensors have been developed recently streamline pathogen immune response detection by addressing limitations traditional methods, including isothermal amplification‐based systems lateral flow assays. This review explores state‐of‐the‐art detecting pathogens, serological assays, biomarkers from material perspective, discussing their advantages, limitations, further potential regarding analytical performance, clinical utility, point‐of‐care adaptability. Additionally, next‐generation biosensing technologies that offer sensitivity selectivity, easy handling end‐users highlighted. An emerging example those powered novel synthetic biology tools, such clustered regularly interspaced short palindromic repeats (CRISPR) with CRISPR‐associated proteins (Cas), combination integrated devices. Lastly, current challenges discussed roadmap furthering advanced manage future pandemics is provided.

Language: Английский

Citations

81
PAM-free loop-mediated isothermal amplification coupled with CRISPR/Cas12a cleavage (Cas-PfLAMP) for rapid detection of rice pathogens DOI

Zaobing Zhu,

Rong Li, Hanwen Zhang

et al.

Biosensors and Bioelectronics, Journal Year: 2022, Volume and Issue: 204, P. 114076 - 114076

Published: Feb. 12, 2022

Language: Английский

Citations

77
Electrochemical biosensor for nucleic acid amplification-free and sensitive detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA via CRISPR/Cas13a trans-cleavage reaction DOI

Woong Heo,

Kyungyeon Lee, Sunyoung Park

et al.

Biosensors and Bioelectronics, Journal Year: 2022, Volume and Issue: 201, P. 113960 - 113960

Published: Jan. 4, 2022

Language: Английский

Citations

74
Recent developments in the use of gold and silver nanoparticles in biomedicine DOI Creative Commons
George Pasparakis

Wiley Interdisciplinary Reviews Nanomedicine and Nanobiotechnology, Journal Year: 2022, Volume and Issue: 14(5)

Published: July 1, 2022

Abstract Gold and silver nanoparticles (NPs) are widely used in the biomedical research both therapeutic sensing/diagnostics fronts. Both metals share some common optical properties with surface plasmon resonance being most exploited property therapeutics diagnostics. Au NPs exhibit excellent light‐to‐heat conversion efficiencies hence have found applications primarily precision oncology, while Ag antibacterial which can be harnessed biomaterials' design. constitute biosensing platforms owing to their plasmonic now routinely various platforms. The utilization of COVID‐19 pandemic was rapidly expanded mostly point‐of‐care extent therapeutics. In this review article, main physicochemical discussed selective examples from recent literature. This article is categorized under: Therapeutic Approaches Drug Discovery > Nanomedicine for Oncologic Disease Diagnostic Tools Vitro Nanoparticle‐Based Sensing Nanotechnology Biology Nanoscale Systems

Language: Английский

Citations

71
CRISPR-Cas12a-Empowered Electrochemical Biosensor for Rapid and Ultrasensitive Detection of SARS-CoV-2 Delta Variant DOI Creative Commons
Chenshuo Wu, Zhi Chen,

Chaozhou Li

et al.

Nano-Micro Letters, Journal Year: 2022, Volume and Issue: 14(1)

Published: Aug. 4, 2022

Coronavirus disease 2019 (COVID-19) is a highly contagious caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The gold standard method for the diagnosis of SARS-CoV-2 depends on quantitative reverse transcription-polymerase chain reaction till now, which time-consuming and requires expensive instrumentation, confirmation variants relies further sequencing techniques. Herein, we first proposed robust technique-methodology electrochemical CRISPR sensing with advantages rapid, sensitivity specificity detection variant. To enhance capability, electrodes are uniformly decorated electro-deposited nanoparticles. Using DNA template identical to Delta spike gene sequence as model, our biosensor exhibits excellent analytical limit (50 fM) high linearity (R2 = 0.987) over six orders magnitude dynamic range from 100 fM 10 nM without any nucleic-acid-amplification assays. can be completed within 1 h stability benefits CRISPR-Cas system. Furthermore, based wireless micro-electrochemical platform, reveals promising application ability in point-of-care testing.

Language: Английский

Citations

70