A comprehensive quality control pipeline in human microbiome research for large population studies DOI Open Access
Ruolin Li, Joost Verlouw, Cindy G. Boer

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 22, 2025

Abstract The widespread application of high-throughput Next Generation Sequencing (NGS) technologies has made microbiome research an emerging field in public health and biomedical sciences. However, there are still many challenges that need to be addressed this field. Pipelines available generate data across cohorts diverse, sources variation recorded evaluated during profiling have not been standardized. Moreover, meticulous quality control the processing, from collection computational quantification is challenging, especially large population studies. Innovative approaches required handle samples minimize potential bias introduced by logistic hurdles biobanking. In paper, we describe methodological steps surrounding optimization 16s rRNA gut two prospective R Study (mean age 9.83 ± 0.32 years) Rotterdam 62.67 5.66 years). This paper also highlights solutions sample mislabeling large-scale analysis. To summarize, our study addresses common problems human research. It aims improve reliability integrating more stringent standards into

Language: Английский

Milk Bioactive Compounds and Gut Microbiota Modulation: The Role of Whey Proteins and Milk Oligosaccharides DOI Creative Commons
Valentina Gallo, Alyexandra Arienzo,

Federica Tomassetti

et al.

Foods, Journal Year: 2024, Volume and Issue: 13(6), P. 907 - 907

Published: March 16, 2024

A strong correlation between the occurrence of various pathological conditions and intestinal dysbiosis is supported by a range evidence. Vice versa, many pathologies have been shown, in turn, to be responsible for alterations gut microbiota, condition that can worsen illness outcomes response therapies. For these reasons, great efforts made, studies are still ongoing, elucidate mechanisms underlying microbiota search pharmacologic or other strategies effectively restore microbiota. In this narrative review, we examined most significant literature on role some milk bioactive compounds, such as oligosaccharides whey proteins, modulating composition action, with aim investigating impact changes mediated molecules human health, their potential use therapeutics treat adjuvate treatment associated pathologies.

Language: Английский

Citations

15
The gut microbiota–brain axis in neurological disorders DOI Creative Commons

Mingming You,

Nan Chen,

Yuanyuan Yang

et al.

MedComm, Journal Year: 2024, Volume and Issue: 5(8)

Published: July 20, 2024

Abstract Previous studies have shown a bidirectional communication between human gut microbiota and the brain, known as microbiota–gut–brain axis (MGBA). The MGBA influences host's nervous system development, emotional regulation, cognitive function through neurotransmitters, immune modulation, metabolic pathways. Factors like diet, lifestyle, genetics, environment shape composition together. Most research explored how regulates host physiology its potential in preventing treating neurological disorders. However, individual heterogeneity of microbiota, strains playing dominant role diseases, interactions these microbial metabolites with central/peripheral systems still need exploration. This review summarizes driving neurodevelopmental disorders (autism spectrum disorder attention deficit/hyperactivity disorder), neurodegenerative diseases (Alzheimer's Parkinson's disease), mood (anxiety depression) recent years discusses current clinical preclinical microbe‐based interventions, including dietary intervention, probiotics, prebiotics, fecal transplantation. It also puts forward insufficient on provides framework for further

Language: Английский

Citations

15
Association of the newly proposed dietary index for gut microbiota and depression: the mediation effect of phenotypic age and body mass index DOI
Xuan Zhang,

Qinglong Yang,

Jingtao Huang

et al.

European Archives of Psychiatry and Clinical Neuroscience, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 8, 2024

Language: Английский

Citations

14
Gut-Brain Axis: Role of Microbiome, Metabolomics, Hormones, and Stress in Mental Health Disorders DOI Creative Commons

Ankita Verma,

Sabra S. Inslicht,

Aditi Bhargava

et al.

Cells, Journal Year: 2024, Volume and Issue: 13(17), P. 1436 - 1436

Published: Aug. 27, 2024

The influence of gut microbiome, metabolites, omics, hormones, and stress on general mental health is increasingly being recognized. Ancient cultures recognized the importance diet overall an individual. Western science modern scientific methods are beginning to unravel foundations mechanisms behind some ancient beliefs customs. organ itself, now thought almost all other organs, ranging from brain reproductive systems. Gut biological sex also a myriad conditions that range disorders, obesity, gastrointestinal cardiovascular diseases health. Here, we review history current understanding gut-brain axis bidirectional talk in various disorders with special emphasis anxiety depressive whose prevalence has increased by over 50% past three decades COVID-19 pandemic biggest risk factor last few years. vagal nerve important contributor this talk, but pathways contribute, most remain understudied. Probiotics containing

Language: Английский

Citations

13
Depression-like phenotypes in mice following common bile duct ligation: Insights into the gut–liver–brain axis via the vagus nerve DOI Creative Commons
Yong Yang, Akifumi Eguchi, Chisato Mori

et al.

Neurobiology of Disease, Journal Year: 2024, Volume and Issue: 192, P. 106433 - 106433

Published: Feb. 7, 2024

Depression frequently occurs in patients with liver cirrhosis, yet the reasons for this correlation are not fully understood. Dysbiosis of gut microbiota has been implicated depression through gut–brain axis via vagus nerve. This study explored potential role gut–liver–brain nerve depression-like phenotypes mice cirrhosis. These underwent common bile duct ligation (CBDL), a method used to stimulate To assess behaviors, behavioral tests were conducted 10 days following either sham or CBDL surgeries. The displayed symptoms such as splenomegaly, elevated plasma levels interleukin-6 and tumor necrosis factor-α, decreased synaptic proteins prefrontal cortex (PFC), disrupted balance, changes blood metabolites (or lipids). Additionally, there positive negative correlations between relative abundance microbiome data Significantly, these reversed by performing subdiaphragmatic vagotomy. Intriguingly, improved after single injection arketamine, new antidepressant. results suggest that CBDL-induced mediated nerve, arketamine might offer treatment approach cirrhosis patients.

Language: Английский

Citations

11
Mood and microbes: a comprehensive review of intestinal microbiota’s impact on depression DOI Creative Commons

Ameer Luqman,

Mei He, Adil Hassan

et al.

Frontiers in Psychiatry, Journal Year: 2024, Volume and Issue: 15

Published: Feb. 9, 2024

Depression is considered a multifaceted and intricate mental disorder of growing concern due to its significant impact on global health issues. The human gut microbiota, also known as the “second brain,” has an important role in CNS by regulating it through chemical, immunological, hormonal, neurological processes. Various studies have found bidirectional link between brain gut, emphasizing onset depression therapies. biological molecular processes underlying microbiota are required, association may represent novel study. However, profound insights into stratification diversity still uncommon. This article investigates emerging evidence bacterial relationship brain’s system potential pathogenicity relevance. interplay immune system, nervous neurotransmitter synthesis, neuroplasticity transitions widely studied. consequences stress, dietary fibers, probiotics, prebiotics, antibiotics GB axis being Multiple revealed this led development effective microbiota-based drugs for both prevention treatment. Therefore, results support hypothesis that influences provide promising area research improved knowledge etiology disease future

Language: Английский

Citations

11
Microbiota-brain axis: Exploring the role of gut microbiota in psychiatric disorders - A comprehensive review DOI
Jawahar Singh,

Vanlallawmzuali,

Amit Kant Singh

et al.

Asian Journal of Psychiatry, Journal Year: 2024, Volume and Issue: 97, P. 104068 - 104068

Published: May 7, 2024

Language: Английский

Citations

10
Circulating B vitamins metabolites in depressive disorders - connections with the microbiota-gut-brain axis DOI

Wiktor Śliwiński,

Oliwia Gawlik-Kotelnicka

Behavioural Brain Research, Journal Year: 2024, Volume and Issue: 472, P. 115145 - 115145

Published: July 9, 2024

Language: Английский

Citations

10
Causal relationship between gut microbiota and hidradenitis suppurativa: a two-sample Mendelian randomization study DOI Creative Commons
Chengling Liu, Xingchen Liu, Xin Li

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: Jan. 29, 2024

Background Accumulating evidence suggests that alterations in gut microbiota composition are associated with the hidradenitis suppurativa (HS). However, causal association between and HS remain undetermined. Methods We performed a bidirectional two-sample Mendelian randomization (MR) analysis using genome-wide study summary data of from MiBioGen consortium which concluded 18,340 individuals analyzed by Consortium, comprising 211 microbiota. were acquired strictly defined collected FinnGenbiobank analysis, included 211,548 European ancestors (409 patients, 211,139 controls). The inverse variance weighted method (IVW), median (WME), simple model, median, MR-Egger used to determine changes pathogenic bacterial taxa, followed sensitivity including horizontal pleiotropy analysis. MR Steiger test evaluated strength leave-one-out assessed reliability results. Additionally, reverse was carried out seek for possible causality. Results By combining findings all steps, we identified four namely, Family XI, Porphyromonadaceae, Clostridium innocuum group Lachnospira. risk might be positively high relative abundance (Odds ratio, OR 2.17, p = 0.00038) Lachnospira (OR 2.45, 0.017) but negatively XI 0.67, 0.049) Porphyromonadaceae 0.29, 0.014). There no noticeable outliers, pleiotropy, or heterogeneity. Furthermore, there proof causation found study. Conclusion This indicates have anti-protective effect on HS, whereas protective HS. Our reveals exists beneficial detrimental offers potentially methods therapy avoidance

Language: Английский

Citations

9
The bidirectional interaction between antidepressants and the gut microbiota: are there implications for treatment response? DOI

Gianluca Borgiani,

Chiara Possidente, Chiara Fabbri

et al.

International Clinical Psychopharmacology, Journal Year: 2024, Volume and Issue: 40(1), P. 3 - 26

Published: Feb. 6, 2024

This review synthesizes the evidence on associations between antidepressant use and gut microbiota composition function, exploring microbiota's possible role in modulating treatment outcomes. Antidepressants exert an influence measures of microbial diversity. The most consistently reported differences were β-diversity those exposed to antidepressants not exposed, with longitudinal studies supporting a potential causal association. Compositional alterations users include increase Bacteroidetes phylum, Christensenellaceae family, Bacteroides Clostridium genera, while decrease was found Firmicutes Ruminococcaceae Ruminococcus genus. In addition, attenuate depressed healthy individuals, modulate serotonin transport, metabolic functions. These lyxose degradation, peptidoglycan maturation, membrane methylerythritol phosphate pathways, alongside gamma-aminobutyric acid metabolism. Importantly, baseline increased α-diversity abundance Roseburia Faecalibacterium are associated response, emerging as promising biomarkers. highlights for predictor response emphasizes need further research elucidate mechanisms underlying antidepressant-microbiota interactions. More homogeneous standardized techniques required confirm these initial findings.

Language: Английский

Citations

9