Lipopolysaccharide Imprinted Polymers for Specific Recognition of Bacterial Outer Membrane Vesicles DOI
Xiaojun Li,

Xiulei Qi,

Xingguo Liu

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(49), P. 19803 - 19811

Published: Nov. 22, 2024

Outer membrane vesicles (OMVs) secreted by bacteria are emerging diagnostic markers for bacterial infection or disease detection due to their carriage of various signaling molecules. However, actual biological samples patients extremely complex, and applying OMVs clinical diagnosis remains a major challenge. One the challenges is that there still great difficulties in enrichment including tedious steps lower concentration. And some commonly used exosome methods, such as ultracentrifugation, have shortcomings. Herein, we introduce lipopolysaccharide (LPS) molecularly imprinted polymer (MIP) efficient capturing analyzing OMVs, enabling novel approach based on biorecognition materials. LPS, unique structure Gram-negative bacteria, also widely expressed surface which will form cyclic hydrogen bonds with functional monomers MIP affinity interactions. The prepared efficiently can isolate from 100 μL culture broth via specific LPS less than 40 min recovery rate over 95%. Moreover, exhibits good reusability, almost identical performance after 5 repeated cycles, contributing reducing experimental costs both time economy. captured be detected using Western blotting target protein antibodies combination proteomic analysis, providing biomarker platform early diagnosis.

Language: Английский

Gastrointestinal and brain barriers: unlocking gates of communication across the microbiota–gut–brain axis DOI Creative Commons
María R. Aburto, John F. Cryan

Nature Reviews Gastroenterology & Hepatology, Journal Year: 2024, Volume and Issue: 21(4), P. 222 - 247

Published: Feb. 14, 2024

Crosstalk between gut and brain has long been appreciated in health disease, the microbiota is a key player communication these two distant organs. Yet, mechanisms through which influences development function of gut–brain axis remain largely unknown. Barriers present are specialized cellular interfaces that maintain strict homeostasis different compartments across this axis. These barriers include epithelial barrier, blood–brain barrier blood–cerebrospinal fluid barrier. ideally positioned to receive communicate microbial signals constituting gateway for gut–microbiota–brain communication. In Review, we focus on how modulation by can constitute an important channel Moreover, malfunction upon alterations composition could form basis various conditions, including often comorbid neurological gastrointestinal disorders. Thus, should unravelling molecular move from simplistic framing as 'leaky gut'. A mechanistic understanding barriers, especially during critical windows development, be aetiology The modulator This Review provides overview examines role disease.

Language: Английский

Citations

92
Unveiling clinical applications of bacterial extracellular vesicles as natural nanomaterials in disease diagnosis and therapeutics DOI Creative Commons
Chun Liu, Negar Yazdani, Corey S. Moran

et al.

Acta Biomaterialia, Journal Year: 2024, Volume and Issue: 180, P. 18 - 45

Published: April 17, 2024

Bacterial extracellular vesicles (BEVs) are naturally occurring bioactive membrane-bound nanoparticles released by both gram-negative and gram-positive bacterial species, exhibiting a multifaceted role in mediating host-microbe interactions across various physiological conditions. Increasing evidence supports BEVs as essential mediators of cell-to-cell communicaiton, influencing pathogenicity, disease mechanisms, modulating the host immune response. However, extent to which these BEV-mediated actions can be leveraged predict onset, guide treatment strategies, determine clinical outcomes remains uncertain, particularly terms their translation potentials. This review briefly describes BEV biogenesis internalisation recipient cells summarises methods for isolation characterization, understanding composition cargo. Further, it discusses potential biofluid-associated biomarkers diseases, spanning cancer non-cancerous Following this, we outline ongoing human trials using vaccine development. In addition diagnostics, this explores emerging research natural or engineered smart nanomaterials applications anti-cancer therapy bone regeneration. discussion extends key factors unlocking BEVs, such standardization characterisation, well other hurdles translating findings setting. We propose that addressing through collaborative efforts well-designed holds fully harnessing BEVs. As field advances, suggests BEV-based nanomedicine has revolutionize management, paving way innovative diagnosis, therapeutics, personalized medicine approaches. STATEMENT OF SIGNIFICANCE: Extracellular (EVs) from bacteria serve multifunctional biomaterials fields biomedicine, bioengineering, biomaterials. majority current studies focus on host-derived EVs, leaving gap comprehensive bacteria-derived EVs (BEVs). Although offer an attractive option drug delivery systems, unique nanostructure easy-to-modify functions make them method diagnosis Our work among pioneering investigating nanobiomaterials plays crucial development diseases therapeutic interventions.

Language: Английский

Citations

26
Unveiling the Wonders of Bacteria-Derived Extracellular Vesicles: From Fundamental Functions to Beneficial Applications DOI Creative Commons

Mariam Rima,

Mariam Dakramanji,

E. Hayek

et al.

Heliyon, Journal Year: 2025, Volume and Issue: 11(4), P. e42509 - e42509

Published: Feb. 1, 2025

:Extracellular vesicles (EVs), are critical mediators of intercellular communication and exhibit significant potential across various biomedical domains. These nano-sized, membrane-encapsulated entities have captured substantial interest due to their diverse roles in pathogenesis promising therapeutic applications. EVs manage numerous physiological processes by transferring bioactive molecules, including proteins, lipids, nucleic acids, between cells. This review delves into the factors influencing properties EVs, such as temperature stress conditions, which collectively influence size, composition, functional attributes. We also describe emerging emphasizing involvement microbial interactions, immune modulation, antimicrobial resistance spread innovative diagnostic instruments. Despite applications, advancement EV-based therapies faces several challenges, will be discussed. By elucidating these elements, we aim provide a comprehensive overview transformative revolutionizing diagnostics therapeutics medicine.

Language: Английский

Citations

4
Bridging gap in the treatment of Alzheimer’s disease via postbiotics: Current practices and future prospects DOI
Bushra Bashir, Monica Gulati, Sukriti Vishwas

et al.

Ageing Research Reviews, Journal Year: 2025, Volume and Issue: unknown, P. 102689 - 102689

Published: Feb. 1, 2025

Language: Английский

Citations

2
Bacterial extracellular vesicles: Vital contributors to physiology from bacteria to host DOI

Xinke Nie,

Qiqiong Li, Xinyang Chen

et al.

Microbiological Research, Journal Year: 2024, Volume and Issue: 284, P. 127733 - 127733

Published: April 25, 2024

Language: Английский

Citations

11
Rapid Biodistribution of Fluorescent Outer-Membrane Vesicles from the Intestine to Distant Organs via the Blood in Mice DOI Open Access
Béatrice Schaack, Corinne Mercier,

Maya Katby

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(3), P. 1821 - 1821

Published: Feb. 2, 2024

A cell's ability to secrete extracellular vesicles (EVs) for communication is present in all three domains of life. Notably, Gram-negative bacteria produce a specific type EVs called outer membrane (OMVs). We previously observed the presence OMVs human blood, which could represent means from microbiota host. Here, order investigate possible translocation intestine other organs, mouse was used as an animal model after administration. To achieve this, we first optimized signal containing fluorescent protein miRFP713 associated with anchoring peptide OmpA by adding biliverdin, fluorescence cofactor, cultures. The miRFP713-expressing produced E. coli REL606 strain were then characterized according their diameter and composition. Native- found homogenous populations vesicles. Finally, vivo ex imaging monitor distribution miRFP713-OMVs mice various organs whether intravenous injection or oral gavage. relative stability signals up 3 days post-injection/gavage paves way future studies investigating OMV-based established between different microbiotas

Language: Английский

Citations

10
Gut microbiome-derived bacterial extracellular vesicles in patients with solid tumours DOI Creative Commons
Surbhi Mishra,

Mysore Vishakantegowda Tejesvi,

Jenni Hekkala

et al.

Journal of Advanced Research, Journal Year: 2024, Volume and Issue: unknown

Published: March 1, 2024

Gut microbiome–derived nanoparticles, known as bacterial extracellular vesicles (bEVs), have garnered interest promising tools for studying the link between gut microbiome and human health. The diverse composition of bEVs, including their proteins, mRNAs, metabolites, lipids, makes them useful investigating diseases such cancer. However, conventional approaches alone may not be accurate in deciphering host–gut communication. In clinical research, there is a gap knowledge on role bEVs solid tumor patients. Analyzing functionality using (meta)genomics proteomics could highlight unique aspects interactions Therefore, we performed comparative analysis proteome microbiota microbiome-derived isolated from patients with tumors healthy controls. After isolating feces controls, spectrometry proteomes next-generation sequencing (NGS) 16S gene. We also investigated microbiomes controls machine learning to classify samples into based fecal microbiomes. Solid showed decreased richness diversity both feces. bEV were more than enriched proteins associated metabolism amino acids carbohydrates, nucleotide binding, oxidoreductase activity. Metadata classification was (100%) compared (93%). Our findings suggest that are functional entities. There need explore together cancer research decipher potential diagnostic or therapeutic biomarkers.

Language: Английский

Citations

9
Bacterial membrane vesicles: orchestrators of interkingdom interactions in microbial communities for environmental adaptation and pathogenic dynamics DOI Creative Commons

Lijun Xiu,

Yuwei Wu,

Gongshi Lin

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: March 21, 2024

Bacterial membrane vesicles (MVs) have attracted increasing attention due to their significant roles in bacterial physiology and pathogenic processes. In this review, we provide an overview of the importance current research status MVs regulating processes, as well crucial environmental adaptation infections. We describe formation mechanism, composition, structure, functions MVs, discuss various Additionally, analyze limitations challenges MV-related prospect potential applications adaptation, mechanisms, novel therapeutic strategies. This review emphasizes significance understanding studying for development new insights into Overall, contributes our intricate interplay between bacteria environment provides valuable strategies targeting pathogenicity.

Language: Английский

Citations

9
Contribution of the seminal microbiome to paternal programming DOI Creative Commons

Justine Kilama,

Carl R Dahlen, Lawrence P. Reynolds

et al.

Biology of Reproduction, Journal Year: 2024, Volume and Issue: 111(2), P. 242 - 268

Published: May 2, 2024

Abstract The field of Developmental Origins Health and Disease has primarily focused on maternal programming offspring health. However, emerging evidence suggests that paternal factors, including the seminal microbiome, could potentially play important roles in shaping developmental trajectory long-term health outcomes. Historically, microbes present semen were regarded as inherently pathogenic agents. this dogma recently been challenged by discovery a diverse commensal microbial community within healthy males. In addition, recent studies suggest transmission semen-associated into female reproductive tract during mating potentials to not only influence fertility embryo development but also contribute offspring. review, we summarize current knowledge microbiota both humans animals followed discussing their potential involvement We propose discuss mechanisms through which influences are transmitted via microbiome. Overall, review provides insights microbiome-based programing, will expand our understanding currently epigenetic modifications, oxidative stresses, cytokines.

Language: Английский

Citations

9
Staphylococcus aureus vesicles impair cutaneous wound healing through p38 MAPK-MerTK cleavage-mediated inhibition of macrophage efferocytosis DOI Creative Commons
Jiaxin Ou,

Kangxin Li,

Hui Yuan

et al.

Cell Communication and Signaling, Journal Year: 2025, Volume and Issue: 23(1)

Published: Jan. 8, 2025

Staphylococcus aureus, a known contributor to non-healing wounds, releases vesicles (SAVs) that influence the delicate balance of host-pathogen interactions. Efferocytosis, process by which macrophages clear apoptotic cells, plays key role in successful wound healing. However, precise impact SAVs on repair and efferocytosis remains unknown. Filtration, ultracentrifugation, iodixanol density gradient centrifugation were used purify bacterial vesicles. Transmission electron microscopy (TEM), nanoparticle tracking analysis (NTA), Western blot (WB) characterize Macrophage efficiency was assessed using flow cytometry confocal microscopy, while at sites analyzed through WB, FACS, TUNEL staining. Hematoxylin eosin (H&E) staining size measurements evaluate healing process. Phosphorylation signaling pathways detected receptor expression measured RNA sequencing, qPCR, cytometry. siRNA pathway inhibitors investigate roles receptors efferocytosis. We identified infected sites, linking them delayed wounds. inhibit activating TLR2-MyD88-p38 MAPK pathway, regulates genes. This activation promoted cleavage shedding MerTK, crucial for macrophage-driven Notably, selective inhibition p38 prevented MerTK shedding, restored accelerated significantly, offering promising therapeutic approach chronic, These findings uncover novel mechanism S. aureus-infected highlighting how disruption via MAPK-MerTK axis becomes force behind impaired Targeting this could open up new avenue facilitating treatment skin injuries.

Language: Английский

Citations

1