Design aspects and characterization of hydrogel-based bioinks for extrusion-based bioprinting

Bioprinting, Journal Year: 2023, Volume and Issue: 32, P. e00274 - e00274

Published: April 15, 2023

3D-bioprinting has become a valid technique for tissue and organ regeneration, as the printing of living cells is allowed while hydrogel-based ink material provides them mechanical structural support. Self-healing shear-thinning hydrogel inks can be considered most promising materials extrusion-based bioprinting (EBB), because extruded due to decrease in viscosity under shear, self-healed after removing which ensures safe shape fidelity bioprinting. To achieve best final result, some technique, biological aspects need considered. In addition, versatile characterization pre- post-printing properties helps improve bioprinted constructs. However, despite great advances 3D-bioprinting, related challenges such opposing characteristics, lack controllable micro-environment, or technological increase speed print resolution must resolved. terms characterization, more standardization also needed. computational modeling would help performance construct. Thus, future going towards larger multifunctional tissue/organ constructs with multi-scale vascularization innervation. Multiple techniques are probably combined, but completely new Further, multimaterial enable heterogeneity gradients On other hand, using 4D-bioprinting, dynamic nature complex organs could added By combining microphysiological platforms (tissue- organ-on-a-chip systems) development functional tissues intended implantation go forward. The translation EBB into clinical practice still early stages, potential regenerative medicine challenges, biomimicry, reproducibility up-scaling issues have been overcome. this review, design property requirements ideal bioink, bioinks presented. Also, prospects discussed.

Language: Английский

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Advancements in bioengineered and autologous skin grafting techniques for skin reconstruction: a comprehensive review

Frontiers in Bioengineering and Biotechnology, Journal Year: 2025, Volume and Issue: 12

Published: Jan. 7, 2025

The reconstruction of complex skin defects challenges clinical practice, with autologous grafts (ASGs) as the traditional choice due to their high graft take rate and patient compatibility. However, ASGs have limitations such donor site morbidity, limited tissue availability, necessity for multiple surgeries in severe cases. Bioengineered (BSGs) aim address these drawbacks through advanced engineering biomaterial science. This study conducts a systematic review describe benefits shortcomings BSGs across wound healing efficacy, integration, immunogenicity, functional outcomes focusing on re-epithelialization, survival, overall aesthetic outcomes. Preliminary findings suggest show superior early results, while demonstrate comparable long-term reduced morbidity. comparative analysis enhances understanding bioengineered alternatives reconstruction, potentially redefining best practices based safety, patient-centric outcomes, highlighting need further innovation solutions.

Language: Английский

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Role of the Extracellular Matrix in Cancer: Insights into Tumor Progression and Therapy

Advanced Therapeutics, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 6, 2025

Abstract The extracellular matrix (ECM) serves not only as a structural scaffold but also an active regulator of cancer progression, profoundly influencing tumor behaviour and the microenvironment (TME). This review focuses into pivotal role ECM alterations in facilitating metastasis explores therapeutic strategies aimed at counteracting these changes. We analyse targeted interventions against collagen, including approaches to inhibit its biosynthesis disrupt associated signalling pathways critical for architecture cell migration. Additionally, therapies addressing hyaluronan are reviewed, highlighting methods suppress synthesis enzymatic degrade it, thereby mitigating tumor‐promoting effects. discussion extends innovative modulating stiffness, focusing on roles cancer‐associated fibroblasts lysyl oxidases, which key contributors remodelling mechanical signalling. By strategically modifying components, aim enhance efficacy existing treatments, tackle resistance mechanisms, achieve more durable outcomes. Insights from recent studies clinical trials highlight promise overcoming treatment improving patient Advancing our understanding biology leads development effective therapies.

Language: Английский

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Collagen as a bio-ink for 3D printing: a critical review

Journal of Materials Chemistry B, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

The significance of three-dimensional (3D) bioprinting in the domain regenerative medicine and tissue engineering is readily apparent.

Language: Английский

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Bioprinting of bespoke islet-specific niches to promote maturation of stem cell-derived islets

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: Feb. 7, 2025

Pancreatic islets are densely packed cellular aggregates containing various hormonal cell types essential for blood glucose regulation. Interactions among these cells markedly affect the glucoregulatory functions of along with surrounding niche and pancreatic tissue-specific geometrical organization. However, stem (SC)-derived generated in vitro often lack three-dimensional extracellular microenvironment peri-vasculature, which leads to immaturity SC-derived islets, reducing their ability detect fluctuations insulin release. Here, we bioengineer vivo-like niches by optimizing combination matrix basement membrane proteins utilizing bioprinting-based guidance recreate spatial pattern islet peripheries. The bioprinted islet-specific promotes coordinated interactions between vasculature, supporting structural functional features resembling native islets. Our strategy not only improves functionality but also offers significant potential advancing research on development, maturation, diabetic disease modeling, future implications translational applications.

Language: Английский

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Recent Tissue Engineering Approaches to Mimicking the Extracellular Matrix Structure for Skin Regeneration

Biomimetics, Journal Year: 2023, Volume and Issue: 8(1), P. 130 - 130

Published: March 22, 2023

Inducing tissue regeneration in many skin defects, such as large traumatic wounds, burns, other physicochemical bedsores, and chronic diabetic ulcers, has become an important clinical issue recent years. Cultured cell sheets scaffolds containing growth factors are already use but have yet to restore normal structure function. Many engineering materials that focus on the process of living tissues been developed for more versatile rapid initiation treatment. Since discovery cells recognize chemical-physical properties their surrounding environment, there a great deal work mimicking composition extracellular matrix (ECM) its three-dimensional network structure. Approaches used ECM constituent proteins well morphological processing methods, fiber sheets, sponges, meshes. This review summarizes material design strategies fields, ranging from morphology existing dressings structures cellular-level microstructure mimicry, explores directions future approaches precision regeneration.

Language: Английский

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Stimuli-responsive chitosan-based nanocarriers for drug delivery in wound dressing applications: A review

Carbohydrate Polymer Technologies and Applications, Journal Year: 2024, Volume and Issue: 7, P. 100497 - 100497

Published: April 6, 2024

Wound care poses a significant global challenge, imposing substantial financial strain on governments across the globe. The exploration of wound dressings (WDs) with potential to enhance healing process has been subject extensive research and scrutiny for an extended period. Traditional dressings, such as bandages, hydrogels, foams, fall short achieving desired (WH) outcomes due their limited ability accurately address dynamic tissue repair. There have suggestions create sophisticated WDs capability interact wounds, perceive alterations in wound's state or surroundings, react correspondingly. This proposal encompasses development advanced that possess interactive features improved monitoring response changes conditions. These innovative incorporate sensors intelligent materials stimuli-responsive self-healing materials, aiming effectively WH. In recent years, there advancement field WDs, introduction smart WDs. cutting-edge include biomechanical mobile injuries, self-removable progressive technology showcases revolutionize management treatment various types wounds. review aims provide overview chitosan nanoparticles (CS NPs) exhibit responsiveness stimuli. article discusses aspects these focusing characteristics applications scientific scholarly manner.

Language: Английский

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3D Bioprinted Human Skin Model Recapitulating Native‐Like Tissue Maturation and Immunocompetence as an Advanced Platform for Skin Sensitization Assessment

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: 13(15)

Published: March 13, 2024

Abstract Physiologically‐relevant in vitro skin models hold the utmost importance for efficacy assessments of pharmaceutical and cosmeceutical formulations, offering valuable alternatives to animal testing. Here, an advanced immunocompetent 3D bioprinted human model is presented assess sensitization. Initially, a photopolymerizable bioink formulated using silk fibroin methacrylate, gelatin photoactivated platelet releasate. The developed shows desirable physicochemical rheological attributes microextrusion bioprinting. tunable physical mechanical properties are modulated through variable photocuring time optimization. Thereafter, utilized bioprint “sandwich type” construct where artificial basement membrane supports biomimetic epidermal layer on one side printed pre‐vascularized dermal other within transwell system. further cultured air–liquid interface maturation. Immunofluorescence staining demonstrated differentiated keratinocyte extracellular matrix (ECM)‐remodeling by fibroblasts endothelial cells. biochemical estimations gene‐expression analysis validate maturation model. incorporation macrophages enhances physiological relevance This effectively classifies irritative non‐irritative substances, thus establishing itself as suitable pre‐clinical screening platform sensitization tests.

Language: Английский

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Toward reproducible tumor organoid culture: focusing on primary liver cancer

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: March 20, 2024

Organoids present substantial potential for pushing forward preclinical research and personalized medicine by accurately recapitulating tissue tumor heterogeneity in vitro . However, the lack of standardized protocols cancer organoid culture has hindered reproducibility. This paper comprehensively reviews current challenges associated with highlights recent multidisciplinary advancements field a specific focus on standardizing liver culture. We discuss non-standardized aspects, including sources, processing techniques, medium formulations, matrix materials, that contribute to technical variability. Furthermore, we emphasize need establish reproducible platforms preserve genetic, proteomic, morphological, pharmacotypic features parent tumor. At end each section, our shifts standardization primary cancer. By addressing these challenges, can enhance reproducibility clinical translation systems, enabling their applications precision medicine, drug screening, research.

Language: Английский

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Nanomaterial strategies in wound healing: A comprehensive review of nanoparticles, nanofibres and nanosheets

International Wound Journal, Journal Year: 2024, Volume and Issue: 21(7)

Published: July 1, 2024

Abstract Wound healing is a complex process that orchestrates the coordinated action of various cells, cytokines and growth factors. Nanotechnology offers exciting new possibilities for enhancing by providing novel materials approaches to deliver bioactive molecules wound site. This article elucidates recent advancements in utilizing nanoparticles, nanofibres nanosheets healing. It comprehensively discusses advantages limitations each these materials, as well their potential applications types wounds. Each despite sharing common properties, can exhibit distinct practical characteristics render them particularly valuable In this review, our primary focus provide comprehensive overview current state‐of‐the‐art applying nanofibres, combinations healing, serving resource guide researchers appropriate utilization nanomaterials wound‐healing research. Further studies are necessary gain insight into application type clinical settings.

Language: Английский

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