Complex probiotics alleviate ampicillin-induced antibiotic-associated diarrhea in mice DOI Creative Commons
Wenwen Li, Siyu Zhang, Yanyan Wang

et al.

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14

Published: April 14, 2023

Antibiotic-associated diarrhea (AAD) is a common side effect during antibiotic treatment, which can cause dysbacteriosis of the gut microbiota. Previous studies have shown beneficial effects in AAD treatment with Bifidobacterium lactis XLTG11, Lactobacillus casei Zhang, plantarum CCFM8661, and rhamnosus Probio-M9. However, no been conducted on immunomodulatory protective intestinal barrier function four complex probiotics. The aim our study to investigate alleviation probiotics ampicillin-induced AAD.Thirty-six BALB/c mice were randomly divided into six groups: normal control group (NC), model (MC), low-, medium-, high-dose groups (LD, MD, HD), positive drug (Bifico, 1 × 107 cfu) (PDC; Bifico, also known as Triple Live Capsule, composed longum, acidophilus, Enterococcus faecalis). An was established by intragastric administration ampicillin, gavage different doses Bifico. weight gain, fecal water content, loose stool grade, permeability, total protein albumin levels, barrier, cytokine microbiota determined.The results showed that significantly decreased ileum tissue damage. Their application increased SIgA, TP, ALB levels. Additionally, levels pro-inflammatory cytokines those anti-inflammatory cytokines. Meanwhile, mRNA expression ZO-1, occludin, claudin-1, MUC2 upregulated probiotic-treated group. Furthermore, diversity modulated changes composition caused ampicillin. At phylum level, abundance Proteobacteria HD lower than MC group, whereas Bacteroidetes higher. genus abundances Klebsiella Parabacteroides lower, Bacteroides, Muribaculaceae, higher Moreover, Spearman's correlation analysis found several specific correlated AAD-related indicators.We improved diarrhea-related indexes, regulated diversity, barrier-related genes, preserved function, relieved inflammation injury, thereby effectively improving AAD-associated symptoms. Graphical Abstract.

Language: Английский

Gut Microbiota, Probiotics, and Their Interactions in Prevention and Treatment of Atopic Dermatitis: A Review DOI Creative Commons
Zhifeng Fang, Lingzhi Li, Hao Zhang

et al.

Frontiers in Immunology, Journal Year: 2021, Volume and Issue: 12

Published: July 14, 2021

Atopic dermatitis (AD) is a public health concern and increasing in prevalence urban areas. Recent advances sequencing technology have demonstrated that the development of AD not only associate with skin microbiome but gut microbiota. Gut microbiota plays an important role allergic diseases including AD. The hypothesis “gut-skin” axis has been proposed cross-talk mechanism between them gradually research. Probiotics contribute to improvement intestinal environment, balance immune responses, regulation metabolic activity. Most studies suggest probiotic supplements may be alternative for prevention treatment This study aimed discuss effects probiotics on clinical manifestation based microbial alterations. Here we reviewed alteration patients AD, association microbiota, epidermal barrier, toll-like receptors, interaction potential mechanisms alleviating via upregulation barrier signaling had discussed, their possible effective substances explored. provides supports targeting attenuate

Language: Английский

Citations

137
Bifidobacterium mechanisms of immune modulation and tolerance DOI Creative Commons
Samuel J. Gavzy, Allison Kensiski,

Zachariah L Lee

et al.

Gut Microbes, Journal Year: 2023, Volume and Issue: 15(2)

Published: Dec. 6, 2023

Bifidobacterium is a widely distributed commensal bacterial genus that displays beneficial pro-homeostatic and anti-inflammatory immunomodulatory properties. Depletion or absence of in humans model organisms associated with autoimmune responses impaired immune homeostasis. At the cellular level, upregulates suppressive regulatory T cells, maintains intestinal barrier function, modulates dendritic cell macrophage activity, dampens Th2 Th17 programs. While there has been large volume literature characterizing probiotic properties various Bifidobacterial species, likely multifactorial mechanisms underlying these effects remain elusive, particular, its tolerogenic effect. However, recent work shed light on surface structural polysaccharide protein elements, as well metabolic products, mediators This review aims to discuss several utilizes for modulation their indirect impact regulation gut microbiome structure from molecules produced metabolites. These are pertinent an increasingly networked understanding tolerance homeostasis health disease.

Language: Английский

Citations

75
Bifidobacterium bifidum Ameliorates DSS-Induced Colitis in Mice by Regulating AHR/NRF2/NLRP3 Inflammasome Pathways through Indole-3-lactic Acid Production DOI

Qingyu Cui,

Zhe Zhang, Xiaoying Tian

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2023, Volume and Issue: 71(4), P. 1970 - 1981

Published: Jan. 12, 2023

In this study, the effectors and mechanisms of Bifidobacterium bifidum FL-276.1 B. FL-228.1 in alleviating dextran sulfate sodium (DSS)-induced colitis were investigated. Both significantly alleviated DSS-induced colitis, whether they supplemented from beginning experiment (whole course intervention) or after DSS induction started (partial intervention). Aryl hydrocarbon receptor (AHR) nuclear factor erythroid 2-related 2 (NRF2) pathways activated mice colons, while NLR family pyrin domain containing 3 (NLRP3) was downregulated under whole intervention modes. Indole-3-lactic acid, an AHR ligand produced by FL-228.1, could regulate AHR/NRF2/NLRP3 pathway Caco-2 monolayers, thus upregulating tight junction proteins protecting integrity epithelial barrier. These results are conducive to promoting clinical trials product development probiotics for colitis.

Language: Английский

Citations

49
Bifidobacterium breve-derived indole-3-lactic acid ameliorates colitis-associated tumorigenesis by directing the differentiation of immature colonic macrophages DOI Creative Commons
Yuke Li, Qingxiang Li,

Ruoshui Yuan

et al.

Theranostics, Journal Year: 2024, Volume and Issue: 14(7), P. 2719 - 2735

Published: Jan. 1, 2024

Aim: To elucidate dynamics and functions in colonic macrophage subsets, their regulation by Bifidobacterium breve (B.breve) its associated metabolites the initiation of colitis-associated colorectal cancer (CAC).Methods: Azoxymethane (AOM) dextran sodium sulfate (DSS) were used to create a CAC model.The tumor-suppressive effect B. variations subsets evaluated.Intestinal macrophages ablated determine role protective effects breve.Efficacious molecules produced identified non-targeted targeted liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis.The molecular mechanism was further verified murine bone marrow-derived (BMDMs), derived from human peripheral blood mononuclear cells (hPBMCs), demonstrated mice.Results: alleviated colitis symptoms, delayed tumorigenesis, promoted phenotypic differentiation immature inflammatory into mature homeostatic macrophages.On contrary, ablation intestinal largely annulled breve.Microbial analysis contents revealed enrichment probiotics depletion potential pathogens following supplementation.Moreover, indole-3-lactic acid (ILA) positively correlated with mice highly enriched culture supernatant breve.Also, addition ILA directly AKT phosphorylation restricted pro-inflammatory response BMDMs hPBMCs vitro.The abolished aryl hydrocarbon receptor (AhR) antagonist CH-223191 or inhibitor MK-2206.Furthermore, could protect against tumorigenesis regulating mice; AhR abrogated relieving tumorigenesis.Conclusion: breve-mediated tryptophan metabolism ameliorates precancerous milieu inhibit directing macrophages.

Language: Английский

Citations

22
Bifidobacterium inhibits the progression of colorectal tumorigenesis in mice through fatty acid isomerization and gut microbiota modulation DOI Creative Commons
Chen Yang,

Huiting Fang,

Haiqin Chen

et al.

Gut Microbes, Journal Year: 2025, Volume and Issue: 17(1)

Published: Feb. 9, 2025

Colorectal cancer (CRC) represents the third most common worldwide. Consequently, there is an urgent need to identify novel preventive and therapeutic strategies for CRC. This study aimed screen beneficial bacteria that have a effect on CRC elucidate potential mechanisms. Initially, we compared gut bacterial metabolites of healthy volunteers patients, which demonstrated intestinal conjugated linoleic acid (CLA), butyric acid, Bifidobacterium in patients were significantly lower than those volunteers, these indicators negatively correlated with Next, spontaneous mouse model conducted explore supplemental CLA-producing Supplementation mice breve CCFM683 B. pseudocatenulatum MY40C prevented Moreover, molecular approaches CLA gene, bbi, key genes prevent Inhibitor intervention results showed PPAR-γ was receptor preventing inhibited NF-κB signaling pathway, up-regulated MUC2, Claudin-1, ZO-1, promoted tumor cell apoptosis via CLA-PPAR-γ axis. Additionally, fecal microbiota transplantation (FMT) metagenomic analysis Odoribacter splanchnicus through production, then by producing up-regulating TJ proteins, regulating cytokines, microbiota. These will contribute clinical trials theoretical research development dietary products.

Language: Английский

Citations

2
Probiotic Bacillus cereus Alleviates Dextran Sulfate Sodium-Induced Colitis in Mice through Improvement of the Intestinal Barrier Function, Anti-Inflammation, and Gut Microbiota Modulation DOI
Kangliang Sheng, Yifan Xu,

Xiaowei Kong

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2021, Volume and Issue: 69(49), P. 14810 - 14823

Published: Oct. 22, 2021

Dysbiosis leads to continuous progress of inflammatory bowel disease (IBD). However, current therapeutic approaches for IBD have limited efficacy and are associated with various side effects. This study focused on exploring the positive effect a new Bacillus cereus (B. cereus) strain (HMPM18123) in colitis mouse model elucidate underlying molecular mechanisms. The symptoms were alleviated by B. administration as evidenced decreased body weight loss, colon length shortening, activity index score, histopathological score. mitigated intestinal epithelial barrier damage upregulating tight junction protein expression. Moreover, exerted anti-inflammatory effects regulating macrophage polarization suppressing TLR4-NF-κB-NLRP3 inflammasome signaling pathways. also rebalanced damaged gut microbiota. Thus, mechanism alleviating treatment involved regulation pathways mucosal barriers modulating microbiota composition.

Language: Английский

Citations

72
Lupeol protects against cardiac hypertrophy via TLR4-PI3K-Akt-NF-κB pathways DOI Open Access
Dan Li, Yingying Guo,

Xian-feng Cen

et al.

Acta Pharmacologica Sinica, Journal Year: 2021, Volume and Issue: 43(8), P. 1989 - 2002

Published: Dec. 16, 2021

Language: Английский

Citations

59
Anti-Inflammatory Activity of Mulberry Leaf Flavonoids In Vitro and In Vivo DOI Open Access
Ziwei Lin, Tiantian Gan,

Yanzhen Huang

et al.

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(14), P. 7694 - 7694

Published: July 12, 2022

Mulberry (Morus alba L.) is a flowering tree traditionally used in Chinese herbal medicine. leaf flavonoids (MLFs) have been reported to exert important anti-inflammatory and antioxidant properties. The purpose of this study was select the MLF with best antioxidative activities from MLFs eluted by different ethanol concentrations (30%, 50%, 75%) explore its pharmacological Three types inhibited production nitric oxide (NO), prostaglandin E2 (PGE2), inducible synthase (iNOS), cyclooxygenase-2 (COX-2), inflammatory cytokines lipopolysaccharide (LPS)-induced RAW 264.7 cells. All boosted capacity decreasing reactive oxygen species (ROS) scavenging 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radicals improving metal ion chelating activity reducing power. results revealed that 30% exhibited activities. A nontargeted metabolomic analysis analyze 24 differential between MLFs. Quercetin, kaempferol, their derivatives 30%MLF were more abundant than other two Furthermore, we evaluated dextran sodium sulfate (DSS)-induced ulcerative colitis (UC) mice. could alleviate clinical symptoms, reduce secretion cytokines, inhibit activation pathway DSS-induced This will provide valuable information for development as functional food.

Language: Английский

Citations

40
Probiotics for Neurodegenerative Diseases: A Systemic Review DOI Creative Commons

Sandhya Ojha,

Nil Patil,

Mukul Jain

et al.

Microorganisms, Journal Year: 2023, Volume and Issue: 11(4), P. 1083 - 1083

Published: April 20, 2023

Neurodegenerative disorders (ND) are a group of conditions that affect the neurons in brain and spinal cord, leading to their degeneration eventually causing loss function affected areas. These can be caused by range factors, including genetics, environmental lifestyle choices. Major pathological signs these diseases protein misfolding, proteosomal dysfunction, aggregation, inadequate degradation, oxidative stress, free radical formation, mitochondrial dysfunctions, impaired bioenergetics, DNA damage, fragmentation Golgi apparatus neurons, disruption axonal transport, dysfunction neurotrophins (NTFs), neuroinflammatory or neuroimmune processes, neurohumoral symptoms. According recent studies, defects imbalances gut microbiota directly lead neurological through gut-brain axis. Probiotics ND recommended prevent cognitive which is major symptom diseases. Many vivo clinical trials have revealed probiotics (Lactobacillus acidophilus, Bifidobacterium bifidum, Lactobacillus casei, etc.) effective candidates against progression ND. It has been proven inflammatory process stress modulated modifying with help probiotics. As result, this study provides an overview available data, bacterial variety, axis defects, probiotics’ mode action averting A literature search on particular sites, PubMed, Nature, Springer Link, identified articles might pertinent subject. The contains following few groups terms: (1) OR (2) disorders. outcomes aid elucidating relationship between effects different neurodegenerative This systematic review will assist discovering new treatments future, as generally safe cause mild side some cases human body.

Language: Английский

Citations

40
Role of Bifidobacterium in Modulating the Intestinal Epithelial Tight Junction Barrier: Current Knowledge and Perspectives DOI Creative Commons
Raz Abdulqadir,

Jessica Engers,

Rana Al–Sadi

et al.

Current Developments in Nutrition, Journal Year: 2023, Volume and Issue: 7(12), P. 102026 - 102026

Published: Oct. 30, 2023

The intestinal tight junction (TJ) barrier is a crucial defense mechanism that prevents the passage of content into wall, tissue, and systemic circulation. A compromised TJ has been identified as significant factor in inflammatory bowel disease (IBD), necrotizing enterocolitis, other gut-related conditions. Recent studies have revealed importance probiotic bacterial strains Bifidobacterium protecting against inflammation IBD pathogenesis via regulation function. Numerous species found to regulate proteins signaling pathways responsible for maintaining integrity permeability. In this review, we provide summary recent highlight regulatory role strain effect on barrier. We also discuss intracellular mechanisms involved modulation potential therapeutic efficacy targeting function inflammation.

Language: Английский

Citations

31