Genetically engineered neural stem cells (NSCs) therapy for neurological diseases; state‐of‐the‐art DOI
Ghufran Lutfi Ismaeel,

Olfet Jabbar Makki AlHassani,

Reem S. Alazragi

et al.

Biotechnology Progress, Journal Year: 2023, Volume and Issue: 39(5)

Published: May 23, 2023

Abstract Neural stem cells (NSCs) are multipotent with remarkable self‐renewal potential and also unique competencies to differentiate into neurons, astrocytes, oligodendrocytes (ODCs) improve the cellular microenvironment. In addition, NSCs secret diversity of mediators, including neurotrophic factors (e.g., BDNF, NGF, GDNF, CNTF, NT‐3), pro‐angiogenic mediators FGF‐2 VEGF), anti‐inflammatory biomolecules. Thereby, transplantation has become a reasonable effective treatment for various neurodegenerative disorders by their capacity induce neurogenesis vasculogenesis dampen neuroinflammation oxidative stress. Nonetheless, drawbacks such as lower migration survival less differential particular cell lineage concerning disease pathogenesis hinder application. Thus, genetic engineering before is recently regarded an innovative strategy bypass these hurdles. Indeed, genetically modified could bring about more favored therapeutic influences post‐transplantation in vivo, making them excellent option neurological therapy. This review first time offers comprehensive capability rather than naïve beyond brain tumors sheds light on recent progress prospect this context.

Language: Английский

Metal–Organic Frameworks‐Based Nanoplatforms for the Theranostic Applications of Neurological Diseases DOI
Huicong Niu, Hui Bu,

Jing Zhao

et al.

Small, Journal Year: 2023, Volume and Issue: 19(23)

Published: March 12, 2023

Abstract Neurological diseases are the foremost cause of disability and second leading death worldwide. Owing to special microenvironment neural tissues biological characteristics cells, a considerable number neurological disorders currently incurable. In past few years, development nanoplatforms based on metal–organic frameworks (MOFs) has broadened opportunities for offering sensitive diagnosis/monitoring effective therapy neurology‐related diseases. this article, obstacles neurotherapeutics, including delayed diagnosis misdiagnosis, existence blood brain barrier (BBB), off‐target treatment, irrepressible inflammatory storm/oxidative stress, irreversible nerve cell summarized. Correspondingly, MOFs‐based diagnostic/monitoring strategies such as neuroimaging biosensors (electrochemistry, fluorometry, colorimetry, electrochemiluminescence, etc.) therapeutic higher BBB permeability, targeting specific lesion sites, attenuation neuroinflammation/oxidative stress well regeneration extensively highlighted management Finally, challenges present research from perspective clinical translation discussed, hoping facilitate interdisciplinary studies at intersections between neurotheranostics.

Language: Английский

Citations

37
Polymeric nanocarriers delivery systems in ischemic stroke for targeted therapeutic strategies DOI Creative Commons
Lin Zhu,

Weijie Zhong,

Xuchen Meng

et al.

Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)

Published: July 18, 2024

Abstract Ischemic stroke is a complex, high-mortality disease with multifactorial etiology and pathogenesis. Currently, drug therapy mainly used treat ischemic in clinic, but there are still some limitations, such as limited blood-brain barrier (BBB) penetration efficiency, narrow treatment time window side effects. Recent studies have pointed out that delivery systems based on polymeric nanocarriers can effectively improve the insufficient for stroke. They provide neuronal protection by extending plasma half-life of drugs, enhancing drug’s permeability to penetrate BBB, targeting specific structures cells. In this review, we classified delivering drugs introduced their preparation methods. We also evaluated feasibility effectiveness discussed existing limitations prospects treatment. hoped review could theoretical basis future development nanomedicine Graphical

Language: Английский

Citations

10
Endogenous Neural Stem Cell–induced Neurogenesis after Ischemic Stroke: Processes for Brain Repair and Perspectives DOI
Hailiang Tang, Yao Li, Weijun Tang

et al.

Translational Stroke Research, Journal Year: 2022, Volume and Issue: 14(3), P. 297 - 303

Published: Sept. 3, 2022

Language: Английский

Citations

30
The Implications of Microglial Regulation in Neuroplasticity-Dependent Stroke Recovery DOI Creative Commons

Chenye Qiao,

Zongjian Liu, Shuyan Qie

et al.

Biomolecules, Journal Year: 2023, Volume and Issue: 13(3), P. 571 - 571

Published: March 21, 2023

Stroke causes varying degrees of neurological deficits, leading to corresponding dysfunctions. There are different therapeutic principles for each stage pathological development. Neuroprotection is the main treatment in acute phase, and functional recovery becomes primary subacute chronic phases. Neuroplasticity considered basis restoration rehabilitation after stroke, including remodeling dendrites dendritic spines, axonal sprouting, myelin regeneration, synapse shaping, neurogenesis. Spatiotemporal development affects spontaneous rewiring neural circuits brain networks. Microglia resident immune cells that contribute homeostasis under physiological conditions. activated immediately phenotypic polarization changes phagocytic function crucial regulating focal global inflammation recovery. We have previously shown neuroplasticity spatiotemporally consistent with microglial activation, suggesting microglia may a profound impact on stroke be key target post-stroke rehabilitation. In this review, we explore as well functions mechanisms polarization, phagocytosis. This followed by summary microglia-targeted rehabilitative interventions influence promote

Language: Английский

Citations

20
Human-induced pluripotent stem cell–derived neural stem cell exosomes improve blood–brain barrier function after intracerebral hemorrhage by activating astrocytes via PI3K/AKT/MCP-1 axis DOI Creative Commons
Conglin Wang, Fangyuan Cheng,

Zhaoli Han

et al.

Neural Regeneration Research, Journal Year: 2024, Volume and Issue: 20(2), P. 518 - 532

Published: April 16, 2024

JOURNAL/nrgr/04.03/01300535-202502000-00029/figure1/v/2024-05-28T214302Z/r/image-tiff Cerebral edema caused by blood-brain barrier injury after intracerebral hemorrhage is an important factor leading to poor prognosis. Human-induced pluripotent stem cell-derived neural cell exosomes (hiPSC-NSC-Exos) have shown potential for brain repair in central nervous system diseases. In this study, we explored the impact of hiPSC-NSC-Exos on preservation and underlying mechanism. Our results indicated that intranasal delivery mitigated neurological deficits, enhanced integrity, reduced leukocyte infiltration a mouse model hemorrhage. Additionally, decreased immune infiltration, activated astrocytes, secretion inflammatory cytokines like monocyte chemoattractant protein-1, macrophage protein-1α, tumor necrosis factor-α post-intracerebral hemorrhage, thereby improving microenvironment. RNA sequencing hiPSC-NSC-Exo PI3K/AKT signaling pathway astrocytes protein-1 secretion, integrity. Treatment with inhibitor LY294002 or neutralizing agent C1142 abolished these effects. summary, our findings suggest maintains part downregulating through activation astrocytes.

Language: Английский

Citations

8
Extracellular vesicles from medicated plasma of Buyang Huanwu decoction-preconditioned neural stem cells accelerate neurological recovery following ischemic stroke DOI Creative Commons
Jun Long, Chenyang Gu, Qiankun Zhang

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2023, Volume and Issue: 11

Published: March 1, 2023

Introduction: The neurological impairment of survivors after ischemic stroke poses a serious risk to their quality life and health. Effective therapeutic options are still lacking. Neural stem cells (NSCs) promote neurogenesis via secreted extracellular vesicles (NSC-EVs), which would be potential option, but the insufficient quantity NSC-EVs in vivo restrains clinical application. Buyang Huanwu Decoction (BHD), classic traditional Chinese medicine (TCM) decoction, is promising alleviate stroke. It was speculated that BHD might recovery through NSC-EVs. Methods: medicated plasma (MP-BHD) prepared precondition NSCs isolate EVs (BHD-NSC-EVs). Middle cerebral artery occlusion (MCAO) models primary were administered evaluate effect. Next-generation sequencing performed explore mechanism. Results: BHD-NSC-EVs more significantly accelerated MCAO promoted proliferation differentiation than alone. MP-BHD enhanced largescale generation BHD-NSC-EVs, encapsulated functional miRNA may play critical roles neurogenesis. Discussion: In replacing or NSCs, preconditioned present efficient strategy for Based on efficacy TCM, preconditioning NSC-derived MP TCM herbs presents newly diseases.

Language: Английский

Citations

14
Biomimetic nanoparticles in ischemic stroke therapy DOI Creative Commons
Zihao Liu, Qian Xia,

Dengzhen Ma

et al.

Discover Nano, Journal Year: 2023, Volume and Issue: 18(1)

Published: March 11, 2023

Abstract Ischemic stroke is one of the most severe neurological disorders with limited therapeutic strategies. The utilization nanoparticle drug delivery systems a burgeoning field and has been widely investigated. Among these, biomimetic composed biogenic membrane components synthetic nanoparticles have extensively highlighted in recent years. Biomimetic camouflage presents an effective strategy to prolong circulation, reduce immunogenicity enhance targeting. For thing, reserve physical chemical properties intrinsic nanoparticle. another, biological functions original source cells are completely inherited. Compared conventional surface modification methods, this approach more convenient biocompatible. In review, membrane-based derived from different donor were exemplified. prospect future ischemic therapy was discussed. Graphic abstract

Language: Английский

Citations

13
A composite hydrogel improves the survival and differentiation of human iPSC-derived neural stem cells after ischemic stroke DOI
Yang Liu, Wen Zhang, Cheng Hu

et al.

Composites Part B Engineering, Journal Year: 2023, Volume and Issue: 259, P. 110711 - 110711

Published: April 6, 2023

Language: Английский

Citations

12
Efficacy and safety of mesenchymal stem cells in patients with acute ischemic stroke: a meta-analysis DOI Creative Commons

Huanjia Huang,

Jian Zhang,

Jinmei Lin

et al.

BMC Neurology, Journal Year: 2024, Volume and Issue: 24(1)

Published: Jan. 29, 2024

Abstract Objective This meta-analysis and systematic review were conducted to comprehensively evaluate the efficacy safety of mesenchymal stem cells in patients with acute ischemic stroke. Method We a manual search electronic databases, including PubMed, Embase, Cochrane Library, Web Science, deadline set for February 1, 2023. Data analysis was performed using Stata version 15.0. Result A total 9 randomized controlled studies included, involving 316 people, 159 147 control groups. Results meta-analysis: Compared placebo group, administration resulted significant reduction National Institutes Health Stroke Scale (NIHSS) scores among diagnosed stroke [SMD=-0.99,95% CI (-1.93, -0.05)]. placebo, barthel index [SMD = 0.48,95% (-0.55,1.51)], modified rankin score 0.45, 95% (1.11, 0.21)], adverse events (RR 0.68, (0.40, 1.17)] difference not statistically significant. Conclusion Based on current studies, cell transplantation can ameliorate neurological deficits certain extent without increasing reactions. However, there no effect Barthel Modified Rankin score.

Language: Английский

Citations

5
Research hotspots and frotiers of stem cells in stroke: A bibliometric analysis from 2004 to 2022 DOI Creative Commons
Qi Zhang,

Yuting Zeng,

Shuqi Zheng

et al.

Frontiers in Pharmacology, Journal Year: 2023, Volume and Issue: 14

Published: March 3, 2023

Background: Stroke is one of the leading causes mortality and permanent disability worldwide. However, current stroke treatment has a limited effect. Therefore, new urgently needed. Stem cell therapy cutting-edge for patients. This study aimed to gain better understanding global stem trends in via bibliometric analysis. Methods: We used Web Science Core Collection search pertinent articles about cells published between 2004 2022. Analysis was conducted using CiteSpace, VOSviewer, R package “bibliometrix” identify publication outputs, countries/regions, institutions, authors/co-cited authors, journals/co-cited journals, co-cited references, keywords. Results: A total 6,703 publications were included The number citations significantly rapidly increased 2022, with most pronounced growth pattern observed period 2008–2009. In terms authoritarian countries, USA had among countries. As institutions prolific institution University South Florida, followed by Oakland then Shanghai Jiao Tong University, Chopp, M. Borlongan, Cesario V, output authors. Regarding Cell Transplantation highest publication, Brain Research . “Mesenchymal as trophic mediators” frequently cited (2,082), article entitled Neuronal replacement from endogenous precursors adult brain after strongest burstiness (strength = 81.35). Emerging hot words past decade “adhesion molecule,” “mesenchymal stromal cell,” “extracellular vesicle,” “pluripotent cells,” “signaling pathway,” “plasticity,” “exosomes.” Conclusion: Between “neurogenesis,” “angiogenesis,” “exosomes,” “inflammation,” “oxidative stress” have emerged research areas on stroke. Although exert positive effects, main mechanisms mitigating damage caused are still unknown. Clinical challenges may include complicating factors that can affect efficacy therapy, which worth deep exploration.

Language: Английский

Citations

11