Therapeutic potential of mesenchymal stem cell‐derived extracellular vesicles: A focus on inflammatory bowel disease DOI Creative Commons
L Clua-Ferré, R Suau,

Irene Vañó‐Segarra

et al.

Clinical and Translational Medicine, Journal Year: 2024, Volume and Issue: 14(11)

Published: Nov. 1, 2024

Abstract Background Mesenchymal stem cell‐derived extracellular vesicles (MSC‐EVs) have emerged as key regulators of intercellular communication, orchestrating essential biological processes by delivering bioactive cargoes to target cells. Available evidence suggests that MSC‐EVs can mimic the functions their parental cells, exhibiting immunomodulatory, pro‐regenerative, anti‐apoptotic, and antifibrotic properties. Consequently, represent a cell‐free therapeutic option for patients with inflammatory bowel disease (IBD), overcoming limitations associated cell replacement therapy, including non‐immunogenic nature, lower risk tumourigenicity, cargo specificity ease manipulation storage. Main Topics Covered This review aims provide comprehensive examination efficacy in IBD, focus on mechanisms action potential impact treatment outcomes. We examine advantages over traditional therapies, discuss methods isolation characterisation, present mechanistic insights into effects through transcriptomic, proteomic lipidomic analyses MSC‐EV cargoes. also available preclinical studies demonstrating reduce inflammation, promote tissue repair restore intestinal homeostasis IBD models, compare these findings those clinical trials. Conclusions Finally, we highlight novel therapy identify challenges opportunities translation practice. Highlights The source mesenchymal cells (MSCs) strongly influences composition function MSC‐derived (EVs), affecting potential. Adipose‐derived MSC‐EVs, known immunoregulatory properties isolation, show promise (IBD). MicroRNAs are consistently across types involved pathways dysregulated making them agents. For example, miR‐let‐7a is inhibition apoptosis, miR‐100 supports survival, miR‐125b helps suppress pro‐inflammatory cytokines miR‐20 promotes anti‐inflammatory M2 macrophage polarisation. Preclinical models shown inflammation suppressing mediators (e.g., TNF‐α, IL‐1β, IL‐6) increasing factors IL‐4, IL‐10). They mucosal healing strengthen integrity gut barrier, suggesting address pathology.

Language: Английский

Harmful Free Radicals in Aging: A Narrative Review of Their Detrimental Effects on Health DOI
Yasser Fakri Mustafa

Indian Journal of Clinical Biochemistry, Journal Year: 2023, Volume and Issue: 39(2), P. 154 - 167

Published: Aug. 1, 2023

Language: Английский

Citations

55
The Role and Prospects of Mesenchymal Stem Cells in Skin Repair and Regeneration DOI Creative Commons
Si Wu,

Shengbo Sun,

Wentao Fu

et al.

Biomedicines, Journal Year: 2024, Volume and Issue: 12(4), P. 743 - 743

Published: March 27, 2024

Mesenchymal stem cells (MSCs) have been recognized as a cell therapy with the potential to promote skin healing. MSCs, their multipotent differentiation ability, can generate various related wound healing, such dermal fibroblasts (DFs), endothelial cells, and keratinocytes. In addition, MSCs neovascularization, cellular regeneration, tissue healing through mechanisms including paracrine autocrine signaling. Due these characteristics, extensively studied in context of burn chronic repair. Furthermore, during investigation unique roles aging scarless also discovered. this review, we summarize by which discuss recent findings from preclinical clinical studies. We explore strategies enhance therapeutic effects MSCs. Moreover, emerging trend combining engineering techniques, leveraging advantages materials, biodegradable scaffolds hydrogels, repair capacity Additionally, highlight using characteristics cell-free therapies future direction cell-based treatments, further demonstrating regenerative aesthetic applications regeneration.

Language: Английский

Citations

24
Therapeutic Applications of Stem Cell-Derived Exosomes DOI Open Access

Omar Abdulhakeem Ahmed Yusuf Abdulmalek,

Khaled Hameed Husain,

Haya Khaled Ali Abdulla AlKhalifa

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(6), P. 3562 - 3562

Published: March 21, 2024

Exosomes are extracellular vesicles of endosomal origin, ranging from 30 to 150 nm in diameter, that mediate intercellular transfer various biomolecules, such as proteins, lipids, nucleic acids, and metabolites. They modulate the functions recipient cells participate diverse physiological pathological processes, immune responses, cell-cell communication, carcinogenesis, viral infection. Stem (SCs) pluripotent or multipotent can differentiate into cell types. SCs also secrete exosomes, which exhibit remarkable therapeutic potential for diseases, especially field regenerative medicine. For example, exosomes derived mesenchymal stem (MSCs) contain miRNAs ameliorate endocrine disorders, diabetes cancer. (sc-exos) may offer similar advantages SCs, but with reduced risks challenges. Sc-exos have lower tumorigenicity, immunogenicity, infectivity. deliver drugs more efficiently penetrate deeper tissues. In this review, we provide an overview recent advances sc-exos their applications We elucidate how biological effects depend on molecular composition. address current challenges future directions using sc-exos.

Language: Английский

Citations

22
Regulation of inflammation during wound healing: the function of mesenchymal stem cells and strategies for therapeutic enhancement DOI Creative Commons

Mingnan Gao,

Han Guo, Xuan Dong

et al.

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: Feb. 15, 2024

A wound takes a long time to heal and involves several steps. Following tissue injury, inflammation is the primary cause of regeneration repair processes. As result, pathophysiological processes involving skin damage, healing, remodeling depend critically on control inflammation. The fact that it feasible target for improving prognosis healing has lately become clear. Mesenchymal stem cells (MSCs) are an innovative effective therapeutic option due their immunomodulatory paracrine properties. By controlling inflammatory milieu wounds through immunomodulation, transplanted MSCs have been shown speed up process. In addition other mechanisms, including handling neutrophil activity modifying macrophage polarization, there may be modifications activation T cells, natural killer (NK) dendritic (DCs). Furthermore, studies pretreating improves ability modulate immunity. this review, we summarize existing knowledge about how influence local in by influencing immunity facilitate We also provide overview optimizing techniques when used treat wounds.

Language: Английский

Citations

18
Stem Cell Therapy for Diseases of Livestock Animals: An In-Depth Review DOI Creative Commons

Raghavendra B. Narasimha,

Sunidhi Shreya, Vijay Anand

et al.

Veterinary Sciences, Journal Year: 2025, Volume and Issue: 12(1), P. 67 - 67

Published: Jan. 17, 2025

Stem cells are unique, undifferentiated that have the ability to both replicate themselves and develop into specialized cell types. This dual capability makes them valuable in development of regenerative medicine. Current stem research has widened their application therapy, drug discovery, reproductive cloning animals, models for various diseases. Although there substantial studies revealing treatment human degenerative diseases using cells, this is yet be explored livestock animals. Many species such as mastitis, laminitis, neuromuscular disorders, autoimmune diseases, some debilitating not covered completely by existing drugs can improved different types like embryonic adult induced pluripotent cells. review mainly focuses on use disease In addition mentioned, potential helpful wound healing, skin genetic disorders. article explores from sources therapy also role conservation endangered well model preparation. Moreover, future perspectives challenges associated with discussed. Overall, transformative impact sector comprehensively studied which will help researchers design work related

Language: Английский

Citations

2
Rheumatoid arthritis: the old issue, the new therapeutic approach DOI Creative Commons
Mahnaz Babaahmadi, Behnoosh Tayebi, Nima Makvand Gholipour

et al.

Stem Cell Research & Therapy, Journal Year: 2023, Volume and Issue: 14(1)

Published: Sept. 23, 2023

Abstract Rheumatoid arthritis (RA) is a chronic and systemic autoimmune disease of unknown etiology. The most common form this inflammatory arthritis, which begins with inflammation the synovial membrane affected joints eventually leads to disability limb. Despite significant advances in RA pharmaceutical therapies availability variety medicines on market, none available medicinal has been able completely cure disease. In addition, percentage (30–40%) patients do not respond appropriately any medicines. Recently, mesenchymal stromal cells (MSCs) have shown promising results controlling diseases, including RA. Experimental studies clinical trials demonstrated high power MSCs modulating immune system. article, we first examine mechanism disease, role cytokines existing therapies. We then discuss immunomodulatory function from different perspectives. Our understanding how work suppressing system will lead better utilization these as tool treatment diseases.

Language: Английский

Citations

30
Triple coumarin-based 5-fluorouracil prodrugs, their synthesis, characterization, and release kinetics DOI
Yasser Fakri Mustafa

Journal of Molecular Structure, Journal Year: 2023, Volume and Issue: 1301, P. 137415 - 137415

Published: Dec. 26, 2023

Language: Английский

Citations

27
Corneal regeneration strategies: From stem cell therapy to tissue engineered stem cell scaffolds DOI Open Access
Mengyuan Wang, Ying Li, Hongqiao Wang

et al.

Biomedicine & Pharmacotherapy, Journal Year: 2023, Volume and Issue: 165, P. 115206 - 115206

Published: July 24, 2023

Corneal epithelial defects and excessive wound healing might lead to severe complications. As stem cells can self-renew infinitely, they are a promising solution for regenerating the corneal epithelium treating injury. The chemical biophysical properties of biological scaffolds, such as amniotic membrane, fibrin, hydrogels, provide necessary signals cell proliferation differentiation. Multiple researchers have conducted investigations on these scaffolds evaluated them potential therapeutic interventions disorders. These studies identified various inherent benefits drawbacks associated with scaffolds. In this study, we provided comprehensive overview history use in repair. We mainly discussed that used transplantation innovative materials under investigation.

Language: Английский

Citations

26
Regulation Mechanisms and Maintenance Strategies of Stemness in Mesenchymal Stem Cells DOI

Nizhou Jiang,

Xiliang Tian, Quanxiang Wang

et al.

Stem Cell Reviews and Reports, Journal Year: 2023, Volume and Issue: 20(2), P. 455 - 483

Published: Nov. 27, 2023

Language: Английский

Citations

23
Current Strategies and Therapeutic Applications of Mesenchymal Stem Cell-Based Drug Delivery DOI Creative Commons

Yasunari Matsuzaka,

Ryu Yashiro

Pharmaceuticals, Journal Year: 2024, Volume and Issue: 17(6), P. 707 - 707

Published: May 30, 2024

Mesenchymal stem cells (MSCs) have emerged as a promising approach for drug delivery strategies because of their unique properties. These include cell membrane-coated nanoparticles, cell-derived extracellular vesicles, immunomodulatory effects, cell-laden scaffolds, and scaffold-free sheets. MSCs offer advantages such low immunogenicity, homing ability, tumor tropism, making them ideal targeted systems. Stem vesicles gained attention immune properties tumor-homing abilities, presenting potential solution challenges. The relationship between MSC-based the self-renewal differentiation capabilities lies in engineered to serve effective carriers therapeutic agents while maintaining intrinsic exhibit potent immunosuppressive functions strategies. EVs immunogenicity strong tissue repair regeneration. Scaffold-free sheets represent cutting-edge regenerative medicine, offering versatile platform engineering regeneration across different medical specialties. shown great clinical applications medicine ability differentiate into various types, secrete bioactive factors, modulate responses. Researchers are exploring these innovative approaches enhance efficiency effectiveness treating diseases.

Language: Английский

Citations

14