Indicaxanthin prevents eryptosis induced by cigarette smoke extract by interfering with active Fas‐mediated signaling DOI
Ignazio Restivo, Ilenia Concetta Giardina, Rosario Barone

et al.

BioFactors, Journal Year: 2024, Volume and Issue: 50(5), P. 997 - 1008

Published: March 23, 2024

Abstract A physiological mechanism of programmed cell death called eryptosis occurs in aged or damaged red blood cells (RBCs). Dysregulated contributes to abnormal microcirculation and prothrombotic risk. Cigarette smoke extract (CSE) induces a p38 MAPK‐initiated, Fas‐mediated eryptosis, activating the death‐inducing signaling complex (DISC). Indicaxanthin (Ind) from cactus pear fruits, is bioavailable dietary phytochemical humans it able incorporate into RBCs enhancing their defense against numerous stimuli. This vitro work shows that Ind, at concentrations mimic plasma after fruit meal, protects erythrocytes CSE‐induced eryptosis. CSE commercial cigarettes was prepared aqueous solution using an impinger air sampler nicotine content determined. were treated with for 3 h absence presence increasing Ind (from 1 5 μM). Cytofluorimetric measurements indicated reduced phosphatidylserine externalization ceramide formation concentration‐dependent manner. Confocal microscopy visualization coimmunoprecipitation experiments revealed prevented both CSE‐triggered Fas aggregation FasL/FADD/caspase 8 recruitment membrane, indicating inhibition DISC assembly. inhibited phosphorylation MAPK, caspase‐8/caspase‐3 cleavage, caspase‐3 activity induced by CSE. Finally, ATP depletion restored aminophospholipid translocase impaired treatment. In conclusion, comparable nutritionally relevant concentrations, can prevent RBC CSE, which suggests intake fruits may limit deleterious effects cigarette smoking.

Language: Английский

JAK-STAT signaling in inflammation and stress-related diseases: implications for therapeutic interventions DOI Creative Commons
Alexey Sarapultsev, Evgeni Gusev, Maria Komelkova

et al.

Molecular Biomedicine, Journal Year: 2023, Volume and Issue: 4(1)

Published: Nov. 8, 2023

Abstract The Janus kinase-signal transducer and transcription activator pathway (JAK-STAT) serves as a cornerstone in cellular signaling, regulating physiological pathological processes such inflammation stress. Dysregulation this can lead to severe immunodeficiencies malignancies, its role extends neurotransduction pro-inflammatory signaling mechanisms. Although JAK inhibitors (Jakinibs) have successfully treated immunological inflammatory disorders, their application has generally been limited diseases with similar pathogenic features. Despite the modest expression of JAK-STAT CNS, it is crucial for functions cortex, hippocampus, cerebellum, making relevant conditions like Parkinson's disease other neuroinflammatory disorders. Furthermore, influence on serotonin receptors phospholipase C implications stress mood This review expands understanding JAK-STAT, moving beyond traditional contexts explore stress-related disorders CNS function. Recent findings, effectiveness Jakinibs chronic rheumatoid arthritis, expand therapeutic applicability. Advances isoform-specific inhibitors, including filgotinib upadacitinib, promise greater specificity fewer off-target effects. Combination therapies, involving monoclonal antibodies, aiming enhance efficacy also give great hope. Overall, bridges gap between basic science clinical application, elucidating complex human health guiding future interventions. Graphical

Language: Английский

Citations

64
Pathophysiology of Red Blood Cell Dysfunction in Diabetes and Its Complications DOI Creative Commons

Alyssa Williams,

Rosi Bissinger,

Hala Shamaa

et al.

Pathophysiology, Journal Year: 2023, Volume and Issue: 30(3), P. 327 - 345

Published: Aug. 2, 2023

Diabetes Mellitus (DM) is a complex metabolic disorder associated with multiple microvascular complications leading to nephropathy, retinopathy, and neuropathy. Mounting evidence suggests that red blood cell (RBC) alterations are both cause consequence of disturbances related DM-associated complications. Importantly, significant proportion DM patients develop varying degrees anemia confounding etiology, increased morbidity. In chronic hyperglycemia, RBCs display morphological, enzymatic, biophysical changes, which in turn prime them for swift phagocytic clearance from circulation. A multitude endogenous factors, such as oxidative dicarbonyl stress, uremic toxins, extracellular hypertonicity, sorbitol accumulation, deranged nitric oxide metabolism, have been implicated pathological RBC changes DM. This review collates clinical laboratory findings hematology indices discusses recent reports on the putative mechanisms underpinning shortened survival disturbed membrane architecture within diabetic milieu. Specifically, death signaling, procoagulant phenotype, RBC-triggered endothelial dysfunction, deformability aggregation context discussed. Understanding provides valuable insights into significance crosstalk between microangiopathy

Language: Английский

Citations

32
Apoptosis and eryptosis: similarities and differences DOI
Anton Tkachenko

APOPTOSIS, Journal Year: 2023, Volume and Issue: 29(3-4), P. 482 - 502

Published: Nov. 30, 2023

Language: Английский

Citations

31
Erythronecroptosis: an overview of necroptosis or programmed necrosis in red blood cells DOI
Anton Tkachenko, Ondřej Havránek

Molecular and Cellular Biochemistry, Journal Year: 2024, Volume and Issue: 479(12), P. 3273 - 3291

Published: March 1, 2024

Language: Английский

Citations

11
Cell death signaling in human erythron: erythrocytes lose the complexity of cell death machinery upon maturation DOI Creative Commons
Anton Tkachenko, Ondřej Havránek

APOPTOSIS, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 9, 2025

Abstract Over the recent years, our understanding of cell death machinery mature erythrocytes has been greatly expanded. It resulted in discovery several regulated (RCD) pathways red blood cells. Apoptosis (eryptosis) and necroptosis share certain features with their counterparts nucleated cells, but they are also critically different particular details. In this review article, we summarize subroutines erythroid precursors (apoptosis, necroptosis, ferroptosis) comparison to (eryptosis erythronecroptosis) highlight consequences organelle clearance associated loss multiple components upon erythrocyte maturation. Recent advances role RCDs health disease have expanded potential clinical applications these lethal subroutines, emphasizing contribution development anemia, microthrombosis, endothelial dysfunction, as well diagnostic biomarkers markers storage-induced lesions. Fas signaling functional caspase-8/caspase-3 system not indispensable for eryptosis, might be retained mediate crosstalk between both erythrocyte-associated RCDs. The ability switch eryptosis suggests that is a simple unregulated mechanical disintegration, tightly controlled process. This allows investigation eventual pharmacological interventions aimed at individual erythrocytes.

Language: Английский

Citations

1
Hemocompatibility studies in nanotoxicology: Hemolysis or eryptosis? (A review) DOI
Anton Tkachenko

Toxicology in Vitro, Journal Year: 2024, Volume and Issue: 98, P. 105814 - 105814

Published: April 4, 2024

Language: Английский

Citations

8
Bufalin reprograms erythrocyte lifespan through p38 MAPK and Rac1 GTPase DOI
Mohammad A. Alfhili, Jawaher Alsughayyir

Toxicon, Journal Year: 2024, Volume and Issue: 240, P. 107636 - 107636

Published: Feb. 3, 2024

Language: Английский

Citations

7
Marine Polysaccharides Carrageenans Enhance Eryptosis and Alter Lipid Order of Cell Membranes in Erythrocytes DOI
Volodymyr Prokopіuk, Anatolii Onishchenko, Liliya Tryfonyuk

et al.

Cell Biochemistry and Biophysics, Journal Year: 2024, Volume and Issue: 82(2), P. 747 - 766

Published: Feb. 9, 2024

Language: Английский

Citations

6
Re-Assessing the Role of Platelet Activating Factor and Its Inflammatory Signaling and Inhibitors in Cancer and Anti-Cancer Strategies DOI Creative Commons
Αλέξανδρος Τσούπρας,

Theodora Adamantidi,

Marios Argyrios Finos

et al.

Frontiers in Bioscience-Landmark, Journal Year: 2024, Volume and Issue: 29(10)

Published: Sept. 26, 2024

Since 2000s, we have outlined the multifaceted role of inflammation in several aspects cancer, via specific inflammatory mediators, including platelet activating factor (PAF) and PAF-receptor (PAFR) related signaling, which affect important junctions cellular interactions that are associated with tumor-related manifestations. It is now well established disease-related unresolved chronic responses can promote carcinogenesis. At same time, tumors themselves able to their progression metastasis, by triggering an inflammation-related vicious cycle, PAF its signaling play crucial role(s), usually conclude tumor growth angiogenesis. In parallel, new evidence suggests also interact cancer treatments inducing antitumor immune response or, conversely, promoting recurrence. Within this review article, current knowledge future perspectives implication all these thoroughly re-assessed. The potential beneficial PAF-inhibitors natural or synthetic modulators PAF-metabolism against tumors, metastasis evaluated. Emphasis given molecules dual anti-PAF anti-cancer activities (Bio-DAPAC-tives), proven potency through clinical trials, as on metal-based anti-inflammatory mediators constitute a class potent inhibitors. way compounds may anti-tumor effects modulate actions discussed. Limitations targeting PAF, metabolism receptor, PAF-related part(s) strategies involve response(s) for improved outcome,

Language: Английский

Citations

5
Anemia and Its Connections to Inflammation in Older Adults: A Review DOI Open Access
Eryk Wacka, Jan Nicikowski, Paweł Jarmużek

et al.

Journal of Clinical Medicine, Journal Year: 2024, Volume and Issue: 13(7), P. 2049 - 2049

Published: April 2, 2024

Anemia is a common hematological disorder that affects 12% of the community-dwelling population, 40% hospitalized patients, and 47% nursing home residents. Our understanding impact inflammation on iron metabolism erythropoiesis still lacking. In older adults, anemia can be divided into nutritional deficiency anemia, bleeding unexplained anemia. The last type might caused by reduced erythropoietin (EPO) activity, progressive EPO resistance bone marrow erythroid progenitors, chronic subclinical pro-inflammatory state. Overall, one-third patients with demonstrate deficiency, have state kidney disease, suffer from unknown etiology. Understanding anemia’s pathophysiology in people aged 65 over crucial because it contributes to frailty, falls, cognitive decline, decreased functional ability, higher mortality risk. Inflammation produces adverse effects cells system. These include (hypoferremia), production, elevated phagocytosis erythrocytes hepatic splenic macrophages. Additionally, causes enhanced eryptosis due oxidative stress circulation. Identifying mechanisms behind age-related essential for better preventing adults.

Language: Английский

Citations

4